中国现代中药2016，Vol.18Issue(2)：143-147,5.DOI:10.13313/j.issn.1673-4890.2016.2.003
流动注射－化学发光法分析延胡索总生物碱
Determination of Alkaloids in Corydalis yanhusuo by Chemiluminescence-Flow Injection Analysis
摘要
Abstract
Objective:To determine the content of alkaloids in Corydalis yanhusuo by Chemiluminescence-Flow Injection Analysis (CL-FIA).Methods:Total alkaloids in the tubers of C.yanhusuo were found to strongly enhance the Chemiluminescence signal of Ce4 +-Rhodamine B system in nitric acid medium.Therefore,a new method was developed for the determination of the alkaloids by CL-FIA,based on the interference test and compared the data of CL-FIA with that of HPLC.Results:Under the optimum conditions,the linear range for the alkaloids was 0.01 4 24-0.227 84 μg·mL -1 (r =0.996 4)and the detection limit was 0.009 06 μg·mL -1 .The average yield was 0.597%(RSD =1 .39%).Conclusion:The established method is more simple, rapid and more sensitive.It is a practical method for detecting total alkaloid in C.yanhusuo.关键词
延胡索/流动注射/化学发光/生物碱/分析检测Key words
Corydalis yanhusuo/Chemiluminescence/Flow-injection/alkaloids/determination引用本文复制引用
朱海萍,史红艳,王仕宝,杨培君..流动注射－化学发光法分析延胡索总生物碱[J].中国现代中药,2016,18(2):143-147,5.基金项目
［基金项目］陕西省教育厅基金项目 （）
