中国现代中药2016，Vol.18Issue(2)：151-155,5.DOI:10.13313/j.issn.1673-4890.2016.2.005
土槿皮乙酸抗肝癌机制研究
Anti-tumor Effect of Pseudolaric Acid B on Hepatocellular Carcinoma HepG2 Cell Line
摘要
Abstract
Objective:The aim of this study was to investigate the anti-tumour role of pseudolaric acid B(PAB)on hepatocellular carcinoma HepG2.Methods:Cell counting analysis confirmed the inhibitory ratio of PAB.The morphological changes were observed by phase contrast microscopy.Scratch test was applied for cell immigration.Phalloidin-Tetramethylrhodamine B isothiocyanate staining was performed for tubulin polymerization.Real time PCR detected the p53 transcription.Western blot was used for protein expression.Results:The inhibitory role of PAB was in time dependent manner.PAB treatment induced the cell death.Cell immigration was inhibited by PAB treatment.Intervening α-tubulin polymerization was visualized after PAB treatment.Real-time PCR test confirmed that PAB treatment inhibited the transcription of p53 gene.And western blot analysis found that PAB regulated the expression of P53 and P27 protein.Conclusion:Therefore PAB had anti-tumor effect on HepG2 through various mechanisms.关键词
土槿皮乙酸/人肝癌 HepG2 细胞/细胞增殖/细胞转移Key words
Pseudolaric acid B(PAB)/hepatocellular carcinoma HepG2/cell proliferation/cell immigration引用本文复制引用
钟婷,薛变变,王增艳,王莹,刘春禹,于静华,胡冬华..土槿皮乙酸抗肝癌机制研究[J].中国现代中药,2016,18(2):151-155,5.基金项目
［基金项目］国家自然科学基金（81301416）；吉林省科技厅重点科技攻关项目（20140204004YY）；国家博士后基金 （）
