中国现代中药2016，Vol.18Issue(2)：208-212,5.DOI:10.13313/j.issn.1673-4890.2016.2.019
大孔树脂对黄芪总皂苷的分离纯化研究
Study on Separation and Purification of Total Saponins from Radix Astragali with Macropere Resin
摘要
Abstract
Objective: To optimize the separation and purification processes of total saponins from Radix Astragali.Methods:The adsorption and desorption properties of total saponins of Astragalus (D1 01 、AB-8、HPD300、HPD600、DM1 30)under static and dynamic conditions were investigated with the content of total saponins as index.Results:The purification effect of HPD300 resin was optimal,when the loading quantities was 2 ∶1 (crude drug of Radix Astragali:macropere Resin),With 70% ethanol as eluant,the amount was 4BV.The Desorption ratio and product purity reached 86.5% and 33.8% separately.Conclusion:The optimized macroporous resin purification process is stable and feasible,and can be used for separation and purification of total saponins of Astragalus.关键词
黄芪/黄芪甲苷/大孔树脂/高效液相色谱-蒸发光散射法Key words
Macropere resin/Radix Astragali/Astragaloside Ⅳ/HPLC-ELSD引用本文复制引用
王登斌,邹准,陈文财,邹节明..大孔树脂对黄芪总皂苷的分离纯化研究[J].中国现代中药,2016,18(2):208-212,5.基金项目
广西科技攻关项目 （）
