中国现代中药2016，Vol.18Issue(2)：219-221,3.DOI:10.13313/j.issn.1673-4890.2016.2.021
赶黄草袋泡茶中黄酮类成分的定量分析
Determination of Flavonoid Constituent in Ganhuangcao Tea Bag by HPLC
杨玲霞 1朱旭江 1刘婷婷2
作者信息
- 1. 甘肃省药品检验研究院，甘肃 兰州 730070
- 2. 西安交通大学第一附属医院 药学部，陕西 西安 710065
- 折叠
摘要
Abstract
Objective: To establish an HPLC method for the determination of quercetin and kaempferol in Ganhuangcao tea bag.Methods:The HPLC analysis was carried out on Kromasil C1 8 (250 mm ×4.6 mm,5 μm)with a mixture of methanol -0.1 % phosphate(50∶50)as the mobile phase.The determination wavelength was set at 360nm with the flow rate of 1 .0 mL·min -1 .Results:The quercetin and kaempferol showed good linearity in the range of 0.2036 ~2.036 0 μg and 0.043 2 ~0.432 0 μg respectively.The average recovery rate of quercetin and kaempferol were 98.43%(RSD =1 .75%) and 97.61 %(RSD =2.1 7%),respectively.Conclusion:The method is simple and accurate.It can be used for the content determination of quercetin and kaempferol in Ganhuangcao tea bag.关键词
赶黄草袋泡茶/槲皮素/山柰酚/高效液相色谱法Key words
Ganhuangcao tea bag/quercetin/kaempferol/HPLC引用本文复制引用
杨玲霞,朱旭江,刘婷婷..赶黄草袋泡茶中黄酮类成分的定量分析[J].中国现代中药,2016,18(2):219-221,3.
