吉林大学学报（医学版）2016，Vol.42Issue(2)：200-203,4.DOI:10.13481/j.1671-587x.20160202
“温阳补气”针法对EAMG大鼠神经肌肉接头处AchR蛋白表达的影响
Effect of“Warming Yang and Nourishing Qi”Acupuncture on acetylcholine receptor protein expression in neuromuscular j unction of rats with EAMG
摘要
Abstract
Objective:To study the influence of “Warming Yang and Nourishing Qi”Acupuncture in the expression levels of acetylcholine receptor protein (AchR)in gastrocnemius neuromuscular junction (NMJ)of the rats with experimental autoimmune myasthenia gravis (EAMG),and to clarify the mechanism of “Warming Yang and Nourishing Qi”Acupucture in the treatment myasthenia gravis (MG).Methods:The female Lewis rats were used to establish the EAMG rat models immuned with AchRα1 129-145 polypeptide fragments.30 EAMG rats were selected randomly and divided into model control group, drug control group, and acupuncture treatment group (n=10),and another 10 rats purchased at the same period were used as blank control group.The rats in drug control group were treated with 1 8.5 mg · kg-1 · d-1 pyridostigmine by gavage. And the rats in acupuncture treatment group were treated with irritating by“Warming Yang and Nourishing Qi”Acupuncture.The rats in blank control group and model control group were not treated .The Lennon symptom scores of the rats in various groups were recorded.After treatment,the tissues of gastrocnemius NMJ of the rats were taken,and the AchR protein expression levels in NMJ of the rats in various groups were detected by Western blotting method. Results:Compared with blank control group,after successfully modelling,the Lennon symptom scores of the rats in model control group, drug control group and acupuncture treatment group before treatment were significantly increased (P<0.01).Compared with model control group,the Lennon symptom scores of the rats in drug control group and acupuncture treatment group after two treatment course were significantly increased (P<0.01 ). Compared with blank control group, the AchR protein expression level in gastronemus NMJ of the rats in model control group was significantly decreased (P<0.01). Compared with model control group, the AchR protein expression levels in gastronemus NMJ of the rats in acupuncture treatment group and drug control group after treatment were significantly increased (P<0.01).Conclusion:“Warming Yang and Nourishing Qi”Acupuncture has good therapeutic effect on the EAMG through increasing the NMJ AchR protein expression level of the EAMG rats.关键词
实验性自身免疫性重症肌无力/温阳补气针法/乙酰胆碱受体蛋白Key words
experimental autoimmune myasthenia gravis/Warming Yang and Nourishing Qi Acupuncture/acetylcholine receptor protein分类
医药卫生引用本文复制引用
王喜臣,沈爱花,胡英华,王洪峰..“温阳补气”针法对EAMG大鼠神经肌肉接头处AchR蛋白表达的影响[J].吉林大学学报（医学版）,2016,42(2):200-203,4.基金项目
国家自然科学基金资助课题（81173344）；教育部博士点科研基金资助课题（20112227120006）；吉林省教育厅重点项目资助课题［吉教科合字（2012第64号）］ （）
