吉林大学学报（医学版）2016，Vol.42Issue(2)：236-239,4.DOI:10.13481/j.1671-587x.20160209
Hedgehog通路对乳腺癌细胞增殖和凋亡的作用及其对Cx32和Cx43表达的影响
Effects of Hedgehog on proliferation and apoptosis of breast cancer cells and its influence in Cx32 and Cx43 expressions
摘要
Abstract
Objective:To study the effects of Hedgehog signal transduction pathway on the cell proliferation, apoptosis and connexin 32 (Cx32)and connexin 43 (Cx43)membranous distribution of breast cancer cells,and to explore its mechanism in the cell proliferation and metastasis of breast cancer.Methods:The breast cancer MCF-7 cells at logarithmic growth period were divided into cyclopamine groups and blank control groups. The MCF-7 in cyclopamine groups were treated with 5,10,20,30 and 40μmol·L-1 for 24,48 and 72 h;MTT assay was applied to detect the inhibitory rate of proliferation of MCF-7 cells. After the MCF-7 cells were treated with 0 (negative control group)and 25μmol·L-1 cyclopamine for 48 h,flow cytometry was employed to determine the apoptotic rate and to analyze membranous distribution of Cx32 and Cx43 in the MCF-7 cells.Results:Compared with blank control group,the inhibitory rates of proliferation in cyclopamine groups were increased (P<0.05), and the inhibitory effect of proliferation was increased with the increasing of cyclopamine doses and prolongation of treatment time.After treated with 25μmol·L-1 cyclopamine,the apoptotic rate of MCF-7 cells was higher than that in blank control group (P<0.05).The positive expression rates of Cx32 and Cx43 48 h after treatment were higher than those in negative control group (P<0.05).Conclusion:Hedgehog signal transduction pathway can inhibit the apoptosis and mediate membranous distribution of Cx32 and Cx43 in breast cancer cells.关键词
Hedgehog通路/乳腺肿瘤/环巴胺/间隙连接蛋白Key words
Hedgehog pathway/breast neoplasms/cyclopamine/connexin分类
医药卫生引用本文复制引用
孟令俊,代恩勇,刁建东,毕林涛,卢振霞..Hedgehog通路对乳腺癌细胞增殖和凋亡的作用及其对Cx32和Cx43表达的影响[J].吉林大学学报（医学版）,2016,42(2):236-239,4.基金项目
国家自然科学基金面上项目资助课题（30870355）；吉林省科技厅科技发展计划项目资助课题 （）
