吉林大学学报（医学版）2016，Vol.42Issue(2)：245-249,5.DOI:10.13481/j.1671-587x.20160211
源于人躯干皮肤真皮的成纤维样细胞的间充质干细胞分化潜能
Potential to differentiation of human dermis-derived fibroblast-like cells into mesenchymal stem cells in vitro
摘要
Abstract
Objective:To study the differentiation capacity of the fibroblast-like cells isolated from human skin dermis into mesenchymal stem cells, and to explore the feasibility to use these cells as alternative cell source of autologus bone marrow mesenchymal stem cells (BMSCs ) for regeneration of tissue inj uries and defects. Methods:Full thickness skin samples were obtained from the abdomen of surgical patients, then digested with dispase and collagenase Ⅰ subsequently. Thereafter, the digested cells were collected and cultured, followed by suspension with serum free medium containing N2,B27,basic fibroblast growth factor (bFGF),and epidermal growth factor (EGF).The skin dermis derived spheroids (SDDSs)were collected and monolayer cultured in serum-containing medium.Finally,the cells were characterized by immunofluorescence staining and differentiation assays.Results:The dermis derived cells proliferated and formed SDDSs in the suspension of serum-free medium. After monolayer cultivation in serum-containing medium, the cells from spheroids were successfully expanded to large number. The cells expressed mesenchymal stem cells markers CD90, CD105 and vimentin. Under osteogenic,chondrogenic and adipogenic differentiation conditions,these cells were differentiated into the alizarin red,safranin O, and oil red O staining positive cells, displayed similar differentiation traits with BMSCs. However,safranin O staining was weaker in the dermis derived cells than BMSCs. Conclusion:A kind of fibroblast-like cells exist in human skin dermis, and have osteocytic, chondrogenic and adipogenic differentiation potentials,demonstrating that these cells will be utilized as a novel cell source for repairing the tissue injury and defect in clinic.关键词
皮肤/真皮/间充质细胞/软骨细胞/骨细胞Key words
skin/dermis/mesenchymal cells/chondrocytes/bone cells分类
医药卫生引用本文复制引用
吕爽,徐金影,冯卫,张泽川,李美英,毕波,孙东杰,李玉林,池光范..源于人躯干皮肤真皮的成纤维样细胞的间充质干细胞分化潜能[J].吉林大学学报（医学版）,2016,42(2):245-249,5.基金项目
教育部回国留学生基金资助课题（3C113H063426）；吉林大学白求恩医学科研支持计划-前沿交叉学科新项目资助课题 （）
