吉林大学学报（医学版）2016，Vol.42Issue(2)：255-259,5.DOI:10.13481/j.1671-587x.20160213
缺血后处理对大鼠肢体缺血再灌注后肺损伤的保护作用及其机制
Protective effect of ischemia postconditioning on lung inj ury after limb ischemia reperfusion in rats and its mechanism
摘要
Abstract
Objective:To observe the effects of ischemic postconditioning (I-postC)on the lung injury after limb ischemia reperfusion (LIR)in the rats,and to investigate the protective effect and the possible mechanisms. Methods:24 Wistar rats were randomly divided into control group, ischemia-reperfusion group (IR group)and I-postC group (n=8 ). Referring to routine method in our department, the model rats which underwent 4 h ischemia and 4 h reperfusion of hind limbs were made.In control group,the rubber band around the limb was loose and the blood flow was not blocked. In I-postC group, before reperfusion, ischemia 5 min and reperfusion 5 min were performed in the rats,repeated for 3 times and then perfusion 4 h was taken,The blood and lung tissue from every rat were taken accurately. The percentages of CD1 8 positive cells in peripheral blood,the levels of soluble intercellular adhesion molecule-1 (sICAM-1)and P-selectin in plasma,the myeloperoxidase (MPO)activities in lung tissue,the levels of intercellular adhesion molecule-1 (ICAM-1)and P-selectin in lung tissue of the rats in various groups were detected. The partial pressure of oxygen (PaO2 ) and partial pressure of carbon dioxide (PaCO2 )were measured.The morphological changes of lung tissue under light and electron microscopes were observed.Results:Compared with control group,the percentage of CD18 positive cells and the levels of sICAM-1 and P-selectin of the rats in IR groups were increased (P<0.01);PaO2 and PaCO2 were decreased significantly;the MPO activity in lung tissue was also significantly increased (P<0.01).The HE staining results showed lung interstitial vascular dilation, congestion, PMN infiltration, the increased gap blood vessel, alveolar septal thickening,alveolar exudation, bronchial epithelial cell shedding and necrosis of the rats in IR group. Compared with IR group,the values of biochemical indicators mentioned above were decreased obviously (P<0.01);PaO2 and PaCO2 were increased significantly (P<0.01);the activities of inflammatory factors in plasma and lung tissue were decreased (P < 0.01 ); the pathological changes of lung damage were improved significantly. Conclusion:I-postC can reduce the lung injury after LIR in the rats,and the mechanism may be related to inhibiting the inflammatory reaction.关键词
缺血后处理/炎症反应/再灌注损伤/肺组织/肢体/大鼠,WistarKey words
ischemic postconditioning/inflammatory reaction/reperfusion inj ury/lung tissue/limb/rats/Wistar分类
医药卫生引用本文复制引用
赵利军,李开济,卢秋玲,门秀丽..缺血后处理对大鼠肢体缺血再灌注后肺损伤的保护作用及其机制[J].吉林大学学报（医学版）,2016,42(2):255-259,5.基金项目
河北省卫生厅医学研究重点项目资助课题 （）
