吉林大学学报（医学版）2016，Vol.42Issue(2)：266-270,5.DOI:10.13481/j.1671-587x.20160215
双氢青蒿素对神经母细胞瘤细胞生长的抑制作用及其机制
Inhibitory effect of dihydroartemisinin on growth of neuroblastoma cells and its mechanism
摘要
Abstract
Objective:To explore the inhibitory effect of dihydroartemisinin (DHA ) on the growth of neuroblastoma cells,and to clarify the anti-tumor mechanism of DHA.Methods:The experiment was divided into blank control group and DHA groups (the final concentrations of DHA were 0.05, 0.50, 5.00 and 50.00μmol·L-1 ).The proliferation rates of neuroblastoma SH-SY5Y cells after treated with DHA were examined by MTT assay;the changes of cell cycle of SH-SY5Y cells after treated with DHA were examined by flow cytometry;the expression levels of cyclin D1 and caspase-3 proteins were detected by ELISA and Western blotting methods.Results:The proliferation of SH-SY5Y cells 24,48,and 72 h after treated with different concentrations of DHA were inhibited.Compared with blank control group,the proliferation rates of SH-SY5Y cells in 0.50,5.00 and 50.00μmol·L-1 DHA groups were significantly decreased (P<0.05 or P<0.01).The density of cells was decreased with the increasing of DHA concentration.Compared with blank control group,the percentage of SH-SY5Y cells at SubG1 phase in 50.00μmol·L-1 DHA group was increased (P<0.05),and the percentage of cells at G0/G1 phase was increased first then was decreased;otherwise, the percentages of cells at S and G2/M phase were decreased.Compared with blank control group,the expression level of cyclin D1 protein in 50.00μmol·L-1 DHA group was decreased (P<0.05),but the expression level of caspase-3 protein in 50.00μmol· L-1 DHA group was increased (P<0.05).Conclusion:DHA could inhibit the proliferation through arresting the cell cycle and inducing the apoptosis of neuroblastoma cells.关键词
双氢青蒿素/神经母细胞瘤/cyclin D1/caspase-3Key words
dihydroartemisinin/neuroblastoma cells/cyclin D1/caspase-3分类
医药卫生引用本文复制引用
齐玲,杨阳,刘玉翠,朱天信,金嵩,臧琳,张玉影,吕鹏,徐冶..双氢青蒿素对神经母细胞瘤细胞生长的抑制作用及其机制[J].吉林大学学报（医学版）,2016,42(2):266-270,5.基金项目
国家自然科学基金资助课题（81201671）；吉林省科技厅科研基金资助课题（20140414049GH）；吉林省卫生厅科研基金资助课题 （）
