草业学报2016，Vol.25Issue(4)：197-203,7.DOI:10.11686/cyxb2015290
纤维素降解菌的筛选及在狼尾草青贮中使用效果评价
Screening of cellulose-degrading bacteria and evaluation of silage performance of Pennisetum
摘要
Abstract
Cellulose-degrading bacteria play important roles in improving forage quality and increasing forage utilization.In this study,a variety of cellulose-degrading bacteria were screened to identify strains with high enzyme activity.Three strains (designated as No.11,No.21,and No.36)were selected from the screening ex-periment and used to produce silage from hybrid Pennisetum.The four treatment groups consisted of treatment 1 (control,CK;hybrid Pennisetum without broth),and treatments 2,3,and 4 (hybrid Pennisetum with broth of strain No.11,21,and 36,respectively).The addition of cellulose-degrading bacteria into silage of hybrid Pennisetum increased the lactic acid content.The lactic acid contents of treatments 2,3 and 4 were 19.42%(P <0.05),38.35% (P <0.05),4.85% (P >0.05)higher,respectively,than that in CK.The neutral deter-gent fiber (NDF),acid detergent fiber (ADF),and hemicellulose (HC)contents of silage were lower in treat-ments 2,3,and 4 than in CK and in the Pennisetum mixtures before ensiling.The silage produced in treatment 3 had the lowest NDF,ADF,and HC contents.The three strains screened from many cellulose-degrading bac-teria improved silage quality,and degraded cellulose to different degrees.Among the three strains,No.21 showed the best performance in degrading cellulose of hybrid Pennisetum and produced silage with the best nu-tritional quality.关键词
纤维素降解菌/酶活力/杂交狼尾草/青贮Key words
cellulose degrading bacteria/enzyme activity/hybrid Pennisetum/silage引用本文复制引用
黄勤楼,钟珍梅,黄秀声,陈钟佃,冯德庆,夏友国..纤维素降解菌的筛选及在狼尾草青贮中使用效果评价[J].草业学报,2016,25(4):197-203,7.基金项目
“十二五”国家科技支撑计划项目（2014BAD15B01，2012BAD14B15-03，2011BAD17B02-06），农业部公益性行业科研专项（201303094-04）和福建省自然基金（2013J01105）资助。 （）
