南方医科大学学报2016，Vol.36Issue(3)：297-302,6.DOI:10.3969/j.issn.1673-4254.2016.03.01
LRG-1在舌癌组织及舌癌细胞系Tca8113中的表达及意义
Expression of LRG-1 in clinical specimens and Tca8113 cell line of tongue carcinoma
摘要
Abstract
Objective To investigate the expression of LRG-1 in clinical specimens and Tca8113 cell line of tongue carcinoma and analyze the relationship between LRG-1 expression and the clinicopathological parameters. Methods LRG-1 expression was detected in 40 tongue squamous cell carcinoma (TSCC) tissues and paired normal adjacent tissues, 20 atypical hyperplasia tissues of the tongue, and 20 tissues of tongue cancer in situ using immunohistochemical method. The expression of LRG-1 in Tca8113 cell line was detected using flow cytometry. The expression of LRG-1 was also detected in human TSCC tissues and Tca8113 cells with Western blotting. The effect of LRG-1 on the proliferation of HUVECs was determined using MTT assay, and its effect on angiogenesis was evaluated with Matrigel tube formation assays. Results Human TSCC tissues had a significantly higher rate of positive expression for LRG-1 (85%, 34/40) than the adjacent tissues (10%, 4/40), invasive tongue cancer (30%, 6/20), and tongue cancer in situ (50%, 10/20) (P<0.05). LRG-1 expression was correlated with the degree of tumor differentiation, clinical stage and lymph node metastasis of the tumor (P<0.05) but not with the patients' age or gender. In the in vitro experiment, LRG-1 promoted HUVEC proliferation and angiogenesis. Conclusion Abnormal LRG-1 expression is present in the human TSCC tissue and Tca8113 cells. LRG-1 can promote HUVEC proliferation and angiogenesis in vitro, suggesting its possible role in promoting tumor angiogenesis.关键词
富亮氨酸α2糖蛋白1/舌鳞癌/Tca8113/血管生成Key words
LRG-1/squamous cell carcinoma of tongue/Tca8113 cells/angiogenesis引用本文复制引用
郝丽静,郑文娇,王淑芬,郑颖,何韶衡,张斌..LRG-1在舌癌组织及舌癌细胞系Tca8113中的表达及意义[J].南方医科大学学报,2016,36(3):297-302,6.基金项目
国家自然科学基金（81471592）；辽宁省教育厅科学研究项目（05L141）Supported by National Natural Science Foundation of China (81471592) （81471592）
