南方医科大学学报2016，Vol.36Issue(3)：309-315,7.DOI:10.3969/j.issn.1673-4254.2016.03.03
新型利福平缓释植入支架材料的制备及体内缓释分析
Fabrication of a new composite scaffold material for delivering rifampicin and its sustained drug release in rats
摘要
Abstract
Objective To fabricate a new composite scaffold material as an implant for sustained delivery of rifampicin and evaluate its performance of sustained drug release and biocompatibility. Methods The composite scaffold material was prepared by loading poly(lactic-co-glycolic) acid (PLGA) microspheres that encapsulated rifampicin in a biphasic calcium composite material with a negative surface charge. The in vitro drug release characteristics of the microspheres and the composite scaffold material were evaluated;the in vivo drug release profile of the composite scaffold material implanted in a rat muscle pouch was evaluated using high-performance liquid chromatography. The biochemical parameters of the serum and liver histopathologies of the rats receiving the transplantation were observed to assess the biocompatibility of the composite scaffold material. Results The encapsulation efficiency and drug loading efficiency of microspheres were (56.05 ± 5.33)% and (29.80 ± 2.88)%, respectively. The cumulative drug release rate of the microspheres in vitro was (94.19 ± 5.4)% at 28 days, as compared with the rate of (82.23±6.28)%of composite scaffold material. The drug-loaded composite scaffold material showed a good performance of in vivo drug release in rats, and the local drug concentration still reached 16.18±0.35μg/g at 28 days after implantation. Implantation of the composite scaffold material resulted in transient and reversible liver injury, which was fully reparred at 28 days after the implantation. Conclusion The composite scaffold material possesses a good sustained drug release capacity and a good biocompatibility, and can serve as an alternative approach to conventional antituberculous chemotherapy.关键词
骨结核/复合支架材料/缓释/生物相容性Key words
osseous tuberculosis/composite scaffold material/sustained drug release/biocompatibility引用本文复制引用
马学铭,林振,张嘉伟,桑朝辉,瞿东滨,江建明..新型利福平缓释植入支架材料的制备及体内缓释分析[J].南方医科大学学报,2016,36(3):309-315,7.基金项目
国家自然科学基金（81272022）Supported by National Natural Science Foundation of China (81272022) （81272022）
