南方医科大学学报2016，Vol.36Issue(3)：316-320,5.DOI:10.3969/j.issn.1673-4254.2016.03.04
八氯代二苯并二噁英对小鼠睾丸组织基因表达谱的影响
Effect of 1, 2, 3, 4, 6, 7, 8, 9-octachlorodibenzo-p-dioxin on testicular gene expression profiles in male mice
摘要
Abstract
Objective To investigate the effect of 1,2,3,4,6,7,8,9-octachlorodibenzo-p-dioxin (OCDD) on the testicular gene expression profile in the testis of mice. Methods Twenty male C57BL/6j mice were randomly divided into normal control group (fed with maize oil) and 3 OCDD groups treated with OCDD by gavage for 30 days at low-, moderate-, and high doses of 1.25×10-6, 1.25 ×10-5, and 1.25 ×10-4 g/mL, respectively (8 mL/kg daily). The testicular gene expression profiles of the mice were investigated using gene chip technique and compared between OCDD-exposed groups and the control group. Results Compared with the control group, the mice in low-dose OCDD group showed 1133 differentially expressed genes, including 659 up-regulated and 474 down-regulated ones;in the moderate-dose OCDD group, 978 genes were differentially expressed, including 487 up-regulated and 491 down-regulated ones; in the high-dose group, 895 genes were differentially expressed, including 424 up-regulated and 471 down-regulated ones. Conclusion The effect of sub-chronic exposure to OCDD on testicular gene expression profiles in male C57BL/6j mice indicates that the testis is probably the target organ of OCDD.关键词
八氯代二苯并二噁英/睾丸/基因表达/基因芯片Key words
1, 2, 3, 4, 6 ,7, 8, 9-octachlorodibenzo-p-dioxin/testis/gene expression/gene chip引用本文复制引用
张伟,王耀峰,倪天军,杨志军..八氯代二苯并二噁英对小鼠睾丸组织基因表达谱的影响[J].南方医科大学学报,2016,36(3):316-320,5.基金项目
国家自然科学基金（81401470）；河南省教育厅自然科学研究计划（13B150216；14B150023）；新乡医学院高学历人才启动基金（505001）Supported by National Natural Science Foundation of China (81401470) （81401470）
