南方医科大学学报2016，Vol.36Issue(3)：321-326,6.DOI:10.3969/j.issn.1673-4254.2016.03.05
miR-181b通过靶向调控N-myc下游调节基因2影响骨肉瘤细胞的迁移和侵袭
microRNA-181b promotes migration and invasion of osteosarcoma cells by targeting N-myc downstream regulated gene 2
摘要
Abstract
Objective To investigate the effects of miR-181b on the migration and invasion of osteosarcoma cells. Methods Three cultured osteosarcoma cell lines and MG-63 cells transfected with miR-181b inhibitor were examined for miR-181b expression using qRT-PCR analysis. The cell migration and invasion of the transfected cells were assessed with Transwell assay. The targets of miR-181b were predicted using a miRNA target prediction software and the results were verified with luciferase reporter assay. The target protein expression in osteosarcoma cells lines was determined by Western blotting, and the cell migration and invasion changes following inhibition of miR-181b or its target protein were assessed using Transwell assay. Results All the 3 osteosarcoma cells lines showed significantly up-regulated miR-181b expression. Inhibition of miR-181b expression obviously suppressed the migration and invasion of MG-63 cells. Based on luciferase reporter assay, N-myc downstream regulated gene 2 (NDRG2) was identified as the direct target gene of miR-181b, and inhibition of NDRG2 expression significantly reversed the effect of miR-181b on cell migration and invasion in MG-63 cells. Conclusion miR-181b is over-expressed in osteosarcoma cells, and inhibition of miR-181b, which directly targets NDRG2, can suppress the migration and invasion of osteosarcoma cells.关键词
miR-181b/骨肉瘤/N-myc下游调节基因2/迁移/侵袭Key words
miR-181b/osteosarcoma cells/N-myc downstream regulated gene 2/cell migration/cell invasion引用本文复制引用
邵建立,李志忠,王亮,焦根龙,周志刚,孙国栋..miR-181b通过靶向调控N-myc下游调节基因2影响骨肉瘤细胞的迁移和侵袭[J].南方医科大学学报,2016,36(3):321-326,6.基金项目
国家自然科学基金（31070862）；广东省中医药局科研课题(20142037)Supported by National Natural Science Foundation of China(31070862) （20142037）
