南方医科大学学报2016，Vol.36Issue(3)：345-350,360,7.DOI:10.3969/j.issn.1673-4254.2016.03.09
磷酸三钙复合骨形态发生蛋白-2/血小板衍生生长因子-BB修复SD大鼠颅骨标准骨缺损
Tricaicium phosphate complex pre-loaded with bone morphogenetic protein-2 or platelet derived growth factor-BB for repairing critical-size cranial defects in SD rats
摘要
Abstract
Objective To observe the effect of a new biomaterial in promoting the bone regeneration for repairing critical-size cranial defects in SD rats. Methods Critical-size cranial defects were induced in 3-month-old male Sprague-Dawley rats and repaired with the implants of calcium phosphate from growth factor enhanced matrix 21 (CaPfromGEM21, control), CaPfromGEM21 preloaded with 10 ng bone morphogenetic protein-2 (BMP-2), CaPfromGEM21 preloaded with 100 ng BMP-2, CaPfromGEM21 preloaded with 0.3μg platelet-derived growth factor-BB (PDGF-BB), or CaPfromGEM21 preloaded with 3μg PDGF-BB. The defects were examined 6 weeks after the surgery with X-ray, micro-CT, HE staining and quantitative assessments. Results X-ray showed defect repair in all the groups. The fracture line became obscure, and the defects were almost fully repaired by the regenerated bone tissues in PDGF-BB group. Micro-CT demonstarted new bone formation in the defects. The new bone volume was significantly greater in PDGF-BB groups than in BMP-2 groups (P<0.05). HE staining revealed the presence of new bones in the defects and new vessels in and around the new bones without inflammatory cells. The new bone area fraction was significantly greater in 10 ng BMP-2 group and 0.3 μg PDGF-BB group than in the control group (P<0.05), and the new vessel density was similar in the all the 4 cytokine-preloaded groups and all significantly greater than that in the blank and CaPfromGEM21 control group (P<0.05). Conclusion CaPfromGem21 combined with BMP-2 or PDGF-BB has good biocompatibility and can better promote bone regeneration for repairing bone defects.关键词
生物材料/CaPfromGEM21/骨形态发生蛋白-2/血小板衍生生长因子/颅骨标准缺损/再生修复Key words
biomaterial/calcium phosphate growth factor enhanced matrix 21/bone morphogenetic protein-2/platelet-derived growth factor-BB/critical-size cranial defects/bone regeneration引用本文复制引用
何瑞轩,肖建斌,宋兵,黄志会,赵亮..磷酸三钙复合骨形态发生蛋白-2/血小板衍生生长因子-BB修复SD大鼠颅骨标准骨缺损[J].南方医科大学学报,2016,36(3):345-350,360,7.基金项目
国家自然科学基金（31328008）；广东省自然科学基金（2014A030313275，S2013010014253）；广东省科技计划项目（2012B010200024）；教育部留学回国人员科研启动项目（31100695）；广州市“珠江科技新星”（2012J2200049）Supported by National Natural Science Foundation of China (31328008) （31328008）
