南方医科大学学报2016，Vol.36Issue(3)：351-355,5.DOI:10.3969/j.issn.1673-4254.2016.03.10
F10基因沉默及过表达对绒癌细胞系JAR细胞周期的影响
Effect of F10 gene silencing and over-expression on cell cycle of choriocarcinoma cell line JAR and the mechanisms
摘要
Abstract
Objective To explore the role of F10 gene in regulating cell cycles of choriocarcinoma cells and the underlying mechanisms. Methods Using untreated cells as the control, JAR cells with F10 gene silencing or stable F10 over-expression were examined for cell cycle changes by flow cytometry (FCM) and for expressions of cyclin and cyclin-dependent kinase (CDKs) with Western blotting and immunofluorescence technique. Results JAR cells over-expressing F10 gene showed reduced duration of cell cycle compared with untreated and with cells after F10 gene silencing. In F10-over-expressing cells, Western blotting revealed significantly up-regulated expressions of cyclin A2, B1, D1, E and CDK2, 6, and 7, but not CDK4, as compared with the control cells and cells with F10 gene silencing (P<0.05), and these results were consistent with those by immunofluorescence assay. Conclusion F10 gene may accelerate cell cycle progression and promote cell proliferation by up-regulating the expressions of cyclin A2, B1, D1, E and CDK 2, 4, 6, 7 in choriocarcinoma cells.关键词
绒癌/F10基因/细胞周期蛋白/CDKs/JAR细胞系Key words
Choriocarcinoma/F10 gene/cyclin/cyclin-dependent kinase/JAR cell引用本文复制引用
杨金娣,庞战军..F10基因沉默及过表达对绒癌细胞系JAR细胞周期的影响[J].南方医科大学学报,2016,36(3):351-355,5.基金项目
国家自然科学基金（81170595）Supported by National Natural Science Foundation of China (81170595) （81170595）
