南方医科大学学报2016，Vol.36Issue(3)：371-374,4.
exendin-4对高糖条件下人系膜细胞细胞外基质代谢的影响
Effects of exendin-4 on extracellular matrix metabolism in human mesangial cells cultured in high glucose
摘要
Abstract
Objective To explore effects of exendin-4 on the metabolism of extracellular matrix (ECM) in human mesangial cells (HMC) cultured in the presence of high glucose and explore the possible mechanism. Methods Human mesangial cells (HMC) were treated with exendin-4 under high glucose conditions. The cell proliferation was observed using CCK8 assay, and the expressions of collagen type I, fibronectin, transforming growth factor-β1 (TGFβ1) expression and extracellular signal-regulated kinase (ERK) signaling pathway activity were assessed using Western blotting. Results Exendin-4 inhibited cell proliferation and the expressions of collagen type I, fibronectin and TGFβ1 and reversed ERK phosphorylation in high glucose-induced HMC. Conclusion Exendin-4 can regulate ECM metabolism in HMC cultured in high glucose by inhibiting TGFβ1/ERK pathway, suggesting the beneficial effects of exendin-4 in preventing and treating diabetic nephropathy.关键词
exendin-4/人系膜细胞/细胞外基质/高糖Key words
exendin-4/human mesangial cell/extracellular matrix/high glucose引用本文复制引用
肖知周,关美萍,郑宗基,贾懿劼,王玲,薛耀明..exendin-4对高糖条件下人系膜细胞细胞外基质代谢的影响[J].南方医科大学学报,2016,36(3):371-374,4.基金项目
国家自然科学基金（31400992）；广东省自然科学基金（2014A030313290）；南方医科大学南方医院院长基金（2013B021）Supported by National Natural Science Foundation of China (31400992) （31400992）
