南方医科大学学报2016，Vol.36Issue(3)：410-413,4.DOI:10.3969/j.issn.1673-4254.2016.03.22
慢性乙型肝炎同病异证发病的分子机制：蛋白质组学分析
The same disease with different syndromes:a proteomic study of chronic hepatitis B
摘要
Abstract
Objective To analyze the differential proteomic profiles of the plasma in patients with chronic hepatitis B presenting with damp-heat retention syndrome and liver stagnation-spleen deficiency syndrome. Methods Two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify the differentially expressed proteins in the plasma of patients with chronic hepatitis B. Results The numbers of plasma proteins detected in healthy volunteers, patients with damp-heat retention syndrome, and patients with liver stagnation-spleen deficiency syndrome were 278 ± 16, 320 ± 14 and 343 ± 19, respectively. Seven differential protein spots were successfully identified by mass spectrum, and were classified into immunological proteins, inflammatory proteins, and lipid metabolism-related proteins. Conclusion In patients with chronic hepatitis B, the multiple differential proteins between damp-heat retention syndrome and liver stagnation-spleen deficiency syndrome suggests the diverse molecular basis of traditional Chinese medicine syndromes, and they might be the molecular tags for different syndromes of the same disease.关键词
慢性乙型肝炎/双向电泳/同病异证/蛋白质组学Key words
chronic viral hepatitis B/two-dimensional electrophoresis/same disease with different syndromes/proteomics引用本文复制引用
陈文莉,罗鸣,陈小苹,黄晶,陈仁..慢性乙型肝炎同病异证发病的分子机制：蛋白质组学分析[J].南方医科大学学报,2016,36(3):410-413,4.基金项目
广东省科学技术厅-广东省中医药科学院联合科研专项 （）
