用于糖链抗原CA19-9实时动态活检的免疫双光子荧光标记试剂盒
Two-photon Fluorescent Labeling Kit for the Real-time Dynamic Biopsy of CA19-9
摘要
Abstract
Carbohydrate antigen CA19-9(Ag) is a tumor marker associated with pancreatic cancer, gallblad-der cancer, colon cancer and gastric cancer in early stage of tumor and cancer stage. Antibody Ab19-9(Ab) to carbohydrate antigens were labeled to form probe-labeled antibody LP-Ab using the prepared two-photon fluorescence probe LP. On the basis of the antigen-antibody specificity affinity principle, LP-Ab could com-bine with carbohydrate antigen Ag into a complex LP-Ab·Ag, which was excited by near infrared laser(740 nm) and emitted strong two-photon fluorescence to accomplish the high sensitive biopsy and real-time dynamic high-resolution imaging of the tumor marker Ag with two-photon fluorescence microscope. Based on this, a simple and convenient two-photon fluorescent labeling kit was prepared to realize the rapid detection of tumor, which is convenient for large-scale clinic testing and screening. The research further broadens the application of LP, and provides a sharp tool for revealing the mechanism of cancer occurrence, development and metasta-sis. LP-Ab can be used to observe and study the dynamic transfer mechanisms of the tumor markers in cells and the mechanism of carcinogenesis.关键词
糖链抗原CA19-9/抗CA19-9单克隆抗体(Ab19-9)/免疫荧光标记/双光子/活检Key words
Carbonhydrate antigen CA19-9/Antibody Ab19-9/Immunofluorescence labeling/Two-photon/Biopsy分类
化学化工引用本文复制引用
黄池宝,张道海,曾伯平,刘其斌,陈华仕,康帅,陈晓远..用于糖链抗原CA19-9实时动态活检的免疫双光子荧光标记试剂盒[J].高等学校化学学报,2016,37(4):638-642,5.基金项目
国家自然科学基金(批准号:21562050, 11464052)、贵州省第八批科技创新人才团队建设专项基金[批准号: 黔科合人才团队(2015)4007]、贵州省科学技术基金项目(批准号: 黔科合J字[2015]2146, [2012]2345)、 贵州省教育厅自然科学重点研究项目(批准号: 黔教合KY字[2014]296, [2013]171)、贵州省教育厅省级本科教学工程建设项目(批准号: 黔教[2014]JXGChcb)、 遵义市"15851人才精英工程"项目[批准号: (2015)4007]和遵义市红花岗区科学技术项目(批准号:遵红科合社字[2015]18)资助. Supported by the National Natural Science Foundation of China(Nos. 21562050, 11464052), the Special Fund Project of the Construction of the Eighth Batch of Scientific and Technological Innovation Talent Team in Guizhou Province, China[No. (2015)4007], Guizhou Provincial Science and Technology Fund Project, China(Nos. J[2015]2146, J[2012]2345), the Key Project of Education Department of Guizhou Province (Nos. KY[2014]296), KY(2013)171), the Teaching Contents and Curriculum System Reform Project of Higher Education in Guizhou Province, China(No. KY [2014] JXGChcb), the Project of "15851 Talents Elite Project" in Zunyi City, China[No. (2015)4007] and the Science and Technology Project of Zunyi City Honghuagang District, China(No. [2015]18). （批准号:21562050, 11464052）
