高等学校化学学报2016，Vol.37Issue(4)：643-647,5.DOI:10.7503/cjcu20150725
用于检测细胞内谷胱甘肽的比率型荧光探针
Ratiometric Fluorescent Probe for the Detection of Glutathione in Living Cells
摘要
Abstract
A fluorescent probe(4) for the detection of biothiols was designed and synthesized and its proper-ties for labeling glutathione was investigated. After reaction with glutathione in 2-[ 4-( 2-hydroxyethyl )-1-piperazinyl] ethanesulfonic acid ( HEPES ) buffer, the color of solution changed from light yellow to pink, which could be detected by naked eyes. Meanwhile, the fluorescence was enhanced at 608 nm. Probe 4 was useful for measuring glutathione at concentrations ranging from 1. 6 × 10-5 to 2 × 10-4 mol/L with a detection limit of 8. 9 ×10-7 mol/L. Besides, probe 4 was sensitive to glutathione in MCF-7 cells, which emitted red fluorescence when it was incubated with MCF-7 cells.关键词
荧光探针/谷胱甘肽/细胞成像Key words
Fluorescent probe/Glutathione/Intracellular imaging分类
化学化工引用本文复制引用
杨润洁,唐尧,朱维平..用于检测细胞内谷胱甘肽的比率型荧光探针[J].高等学校化学学报,2016,37(4):643-647,5.基金项目
国家自然科学基金(批准号:21476077)资助. Supported by the National Natural Science Foundation of China(No. 21476077). （批准号:21476077）
