肝胆胰外科杂志2016，Vol.28Issue(2)：98-101,4.DOI:10.11952/j.issn.1007-1954.2016.02.003
miR-1246在肝癌组织中的表达及其对人肝癌细胞增殖和凋亡的影响
Expression of miR-1246 in human hepatocellular carcinoma and its effect on cell proliferation and apoptosis
摘要
Abstract
objective To observe the expression of miR-1246 in human hepatocellular carcinoma tissues and its effect on cell proliferation and apoptosis. Methods The expression of miR-1246 in hepatocellular carcinoma tissues and normal liver tissues was detected by real-time PCR (qRT-PCR). The cultured hepatocellular carci-noma cell line BEL7402 and SMMC7721 cells were transfected with miR-1246 inhibitor, the cell proliferation was detected by MTT assay and apoptosis was analyzed by flow cytometry. Results The expression of miR-1246 in hepatocellular carcinoma tissues was significantly higher than that in normal liver tissues [(65.39±8.77) vs (10.23±2.58), P=0.002)]. After transfected miR-1246 inhibitor, BEL7402 cell transfected hepatocellular carci-noma cells showed low proliferation rate and high apoptosis rate when compared with control group (P<0.05), the same trend was also observed in SMMC7721 cells (P<0.05). Conclusion These results indicate that the miR-1246 is highly expressed in hepatocellular carcinoma tissues and its high expression may be involved in the de-velopment of hepatocellular carcinoma.关键词
微小RNA-1246/癌,肝细胞/增殖/凋亡Key words
microRNA 1246 (miR-1246)/hepatocellular carcinoma/proliferation/apoptosis分类
医药卫生引用本文复制引用
彭兆意,周喜洋..miR-1246在肝癌组织中的表达及其对人肝癌细胞增殖和凋亡的影响[J].肝胆胰外科杂志,2016,28(2):98-101,4.基金项目
浙江省台州市科技项目（100KY-58）。 （）
