肝胆胰外科杂志2016，Vol.28Issue(2)：117-120,4.DOI:10.11952/j.issn.1007-1954.2016.02.008
蛋白酶体抑制剂MG132对胆囊癌细胞杀伤作用的实验研究
Experimental study of the killing effect of proteasome inhibitor MG132 on gallbladder cancer cells
摘要
Abstract
objective To investigate the mechanism of proteasome inhibitor MG132 in inhibiting prolifera-tion and inducing apoptosis of gallbladder cancer cells. Methods The anti-tumor activity of MG132 on gallblad-der cancer cells was assessed by the CCK8 assay. Apoptosis changes were detected by flow cytometric analysis. The protein and mRNA expression of Caspase-8, Caspase-3 and DR5 were examined using Western blotting and RT-PCR. Gallbladder cancer xenografts in athymic nude mice were established and the tumor weight and volume were measured after the intervention of MG132. Results The results showed that MG132 inhibited the prolifera-tion of gallbladder cancer cells in a dose-dependent manner both in vivo and in vitro (P<0.01). Exposure of tumor cells to MG132 induced cell cycle arrest at G2/M phase and apoptosis in a dose- dependent manner (P<0.01). In addition, analysis of apoptotic pathways indicated that MG132 significantly enhanced the activation of Caspase-8, Caspase-3 and DR5 in extrinsic signaling pathway (P<0.05). Conclusion Proteasome inhibitor MG132 can ef-fectively kill gallbladder cancer cells and its mechanism involves the cell cycle arrest and apoptosis induction.关键词
胆囊癌/蛋白酶体抑制剂/增殖/凋亡Key words
gallbladder carcinoma/proteasome inhibitor/proliferation/apoptosis分类
医药卫生引用本文复制引用
朱卫平,詹棣华,赵一鸣,王鲁,解宏伟,王会鹏,程志俭..蛋白酶体抑制剂MG132对胆囊癌细胞杀伤作用的实验研究[J].肝胆胰外科杂志,2016,28(2):117-120,4.基金项目
国家自然科学基金项目（81372314，81272393）；上海市卫生局青年基金项目（20134Y089）；上海市闵行区自然科学基金项目（2012MHZ025）；复旦大学附属上海市第五人民医院院内课题（2010QJ05）。 （）
