肝胆胰外科杂志2016，Vol.28Issue(2)：125-128,132,5.DOI:10.11952/j.issn.1007-1954.2016.02.010
不同时机早期肠内营养对重症急性胰腺炎大鼠肠黏膜屏障的影响
Effect of early enteral nutrition at different periods on intestinal mucosa barrier in rats with se-vere acute pancreatitis
摘要
Abstract
objective To investigate the effect of early enteral nutrition (EN) at different period on intestinal mucosa barrier and pathology in rats with severe acute pancreatitis (SAP). Methods A total of 75 male rats were randomly allocated into following groups: blank group (S group, n=15), experimental group (EN group, 45 rats were then randomly divided into EN-1, EN-2 and EN-3 sub group, each n=15) and control group (TPN group, n=15). Rats in S group only had their viscera turned, while rats in other two groups were used to establish severe acute pancreatitis model. After successful modeling, the S group received normal diet, the TPN group received support and treatment by total parenteral nutrition. EN-1, EN-2 and EN-3 subgroup respectively started enteral nutrition at day 1, day 2 and day 4 after modeling. At the day 2, day 3 and day 5 after modeling, 5 rats in each group were sacrificed. Then the heart blood sampling were taken to detect serum levels of amylase (AMS), tumor necrosis factor-α (TNF-a), interleukin-10 (IL-10) and endotoxin (ET), and the terminal ileum tissue was taken for histopathologic examination and evaluation. Results At day 2, compared with EN subgroups and TPN group, indexes in S group were significantly different (P<0.01), and there was no significant difference between EN groups and TPN group (P>0.05). At day 3 and day 5, indexes in EN group were lower than those in TPN group, and these four groups showed a downward trend along with the treatment (P<0.05). The EN-1 group decreased most significantly. Conclusion Starting early enteral nutrition can control the progress of SAP in rats, and protect intestinal mucosal barrier, the sooner the better.关键词
重症急性胰腺炎/肠内营养/肠黏膜屏障/大鼠Key words
severe acute pancreatitis/enteral nutrition/intestinal mucosa barrier/rats分类
医药卫生引用本文复制引用
李峰,徐传博,夏红星,谢荣俊,王辉..不同时机早期肠内营养对重症急性胰腺炎大鼠肠黏膜屏障的影响[J].肝胆胰外科杂志,2016,28(2):125-128,132,5.基金项目
湖南省教育厅医学科研项目（09C848）。 （）
