国际医药卫生导报2016，Vol.22Issue(8)：1050-1054,5.DOI:10.3760/cma.j.issn.1007-1245.2016.08.004
miRNA-221对肝癌HepG2细胞增殖及凋亡的影响
Effect of miRNA-221 on proliferation and apoptosis of hepatocellular carcinoma HepG2 cells
韩双喜 1王丽 2王介营 1李传光 1张锋刚1
作者信息
- 1. 251700 滨州市中心医院普通外科
- 2. 251700 滨州市中心医院儿科
- 折叠
摘要
Abstract
Objective To study the effect of lentivirus-mediated miRNA-221 RNAi on the biological behaviors of human hepatic cancer cells.Methods miRNA-221 specific siRNA gene was synthesized and cloned into the recombinant lentiviral vector pGCSIL-GFP.Hepatic cancer HepG2 cells were infected by microRNA-22 1-siRNA recombinant lentivirus(miRNA-221-siRNA-LV),which were set as an SI group.The cells infected with miRNA-221-NC were set as an NC group.And the non-infected cells were set as an N group.The expression of the targeted genes of miRNA-221 were detected by transcription polymerase chain reaction (RT-PCR).Cell proliferation was analyzed by MTT assay.Apoptosis was detected by Hoechst33258 and the apoptosis gene Caspase 3 was detect by RT-PCR.The invasion was detected by transwell method.BALB/c nude mice were subcutaneously transplanted with HepG2 cells.Results (1) miRNA-221-siRNA-LV could significantly lowered the expression of miRNA-221.(2) Hour 96 of MTT experiment,the absorbance was markedly lower in the SI group than in the NC group and the N group (P=-0.003 2).(3) The number of cells that migrated through the chamber of the SI group was smaller than those of the NC group and the N group(P=0.000 4).(4)The apoptosis was higher in the SI group than in the NC group and the N group.In addition,the expression of caspase 3 mRNA was significantly higher in the SI group than in the NC group and the N group (P=0.000 2).Conclusions miRNA-221-siRNA suppresses the proliferation and migration of HepG2 cells and promote HepG2 cells'apoptosis by lowering the expression of miRNA-221.关键词
miRNA-221/慢病毒/肝癌/HepG2Key words
miRNA-221/Lentivirus/Hepatocellular carcinoma/HepG2引用本文复制引用
韩双喜,王丽,王介营,李传光,张锋刚..miRNA-221对肝癌HepG2细胞增殖及凋亡的影响[J].国际医药卫生导报,2016,22(8):1050-1054,5.
