广西植物2016，Vol.36Issue(3)：342-348,7.DOI:10.11931/guihaia.gxzw201408021
龙南钾矿区常见蕨类植物可培养内生真菌的多样性
Diversity of culturable endophytic fungi separated from seven common ferns in potassium mine areas in Longnan, South Jiangxi Province
摘要
Abstract
Plant endophytic fungi is a special kind of microorganisms mutualistic ally symbiosed with host plants. The special microbial resources have attracted tremendous interests in recent years, because of their potential to address problems associated with enhanced plant growth in environmental stress of mines and to improve the efficiency of ecologi-cal restoration. With a long peoriod of evolution, endophytic fungi and their host plants have formed a closemutual symbi-otic relationship. Many beneficial biological effects have been made on the host plants, and they have the important eco-logical significance in plant community succession process. It is widely attracted using plants-microorganisms to enhance plant growth in environmental stress of mines, and to improve biological restoration efficiency, and its significance to host plants has become a new hot issue. In order to make clear the species diversity, community composition and ecologi-cal distribution of endophytic fungi, we carried out our work among 7 species of common ferns in potassium mine areas in Longnan, South Jiangxi Province. They are Dicranopteris linearis, Woodwardia japonica, Athyrium yokoscense, Stenoloma chusanum, Lygodium japonicum, Asplenium sarelii and Pteris multifida. And we analyzed the diversity of endophytic fun-gi, using the method of tissue isolation, morphological identification. The results showed that 377 strains of endophytic fungi were isolated from 7 species of plants, and the separation rate ranged from 2.50%to 4.52%. All of 377 strains be-long to 25 genera, and among them, Alternaria, Aspergillus, Cladosporium and Verticillium,which are found in all these 7 fern species, indicated that they were dominant, but there were obvious differences in the distribution in each fern spe-cies. The total separation rate and the colonization rate of entophytic fungi from leaves are generally greater than that from the rhizomes, but there are some differences in the total separation rate and the colonization rate from different parts of each fern species. The diversity indexes of endophytic fungi range from 0.502 to 0.867 in the seven species of ferns. It in-dicated that there was high diversity of endophytic fungi in potassium mines and the low similarity of endophytic fungi in different ferns in the same mine areas. The similarity coefficients of the endophytic fungi in the seven species of ferns were from 0.189 to 0.587. The results of this study would not only enrich germplasm resources of plant endophytic fungi, but also would offer basic data for further research on the mechanism that endophytic fungi could strengthen the adapta-tion of the host plant on mineral nutrition and stress physiology in potassium mine areas.关键词
钾矿区/蕨类植物内生真菌/优势菌群/多样性指数/相似性系数Key words
potassium mine areas/endophytic fungi in ferns/dominant endophytic fungi/diversity index/similarity coefficient分类
生物科学引用本文复制引用
钱旭,甘会云,杜勇涛,张鲜,陈晔,樊有赋..龙南钾矿区常见蕨类植物可培养内生真菌的多样性[J].广西植物,2016,36(3):342-348,7.基金项目
国家自然科学基金(31360064,51264014)@@@@[Supported by the National Natural Science Foundation of China(31360064,51264014)]。 （31360064,51264014）
