解放军医药杂志2016，Vol.28Issue(4)：5-8,4.DOI:10.3969/j.issn.2095-140X.2016.04.002
医用创面胶和吻合胶对小鼠成纤维细胞L929毒性的研究
A Cytotoxicity Study on Medical Wound Adhesive and Anastomotic Adhesive on Fibroblast L929 Cell of Mice
摘要
Abstract
Objective To investigate the indirect cytotoxicity of medical wound adhesive and anastomotic adhe-sive on fibroblast cells of mice. Methods The cytotoxicity of the two kinds of adhesives on fibroblast L929 cells of mice was detected. The effects of two kinds of medical adhesives on cell activity were detected using CCK-8 method;the effect on cell division and growth was detected using 4',6-diamidino-2-phenylindole ( DAPI) fluorescence staining method, and fluorescein isothiocyanate/propidium iodide ( FITC/PI) apoptosis staining method was used to detect the effect on apopto-sis. Results There was no significant difference in fibroblasts L929 activity of mice in the medical wound adhesive and anastomotic adhesive groups ( P>0. 05 ) , but the reduced values in the two groups were lower than that in the control group (P<0. 05). There were no significant differences in fluorescence staining cell count, total area proportion and sur-vival rate of normal cells among the three groups (P>0. 05). Conclusion The medical wound and anastomotic adhe-sives may significantly reduce the activity of peripheral adjacent cells with the similar reduction degree. However, they can not stop the normal of cells growth of cells and can not induce apoptosis.关键词
创面胶/吻合胶/细胞毒性/小鼠成纤维细胞Key words
Wound adhesive/Anastomotic adhesive/Cytotoxicity/Fibroblasts cell/mouse分类
医药卫生引用本文复制引用
余宽宽,梁向党,孙赓,房卓群,郭占芳,王歆淏..医用创面胶和吻合胶对小鼠成纤维细胞L929毒性的研究[J].解放军医药杂志,2016,28(4):5-8,4.基金项目
军队重点创新专项(13CXZ032) （13CXZ032）
