解放军医药杂志2016，Vol.28Issue(4)：9-11,3.DOI:10.3969/j.issn.2095-140X.2016.04.003
新型神经吻合口制动动物模型的建立
Establishment of New Animal Models of Peripheral Nerve Anastomosis Brake
摘要
Abstract
Objective To establish the experimental animal model of braking nerves in order to investigate the effect of mechanical stress on the nerve regeneration following peripheral nerve injury. Methods A total of 24 SD rats were randomly divided into experimental group (n=12) and control group (n=12). The models of neural injury were estab-lished in the two groups. The experimental group was covered with silica gel tube in distal segment of sciatic nerves, and then was fixed with medical adhesive on the two sides of the tube;end-to-end suture of sciatic nerves was performed in con-trol group. The effect of breaking nerve anastomosis was evaluated, the results of electrophysiological examination and nerve anastomosis histopathology 8 weeks after the operation in the two groups were observed. Results The nerve anastomosis completely braked without displacement in experimental group. The results of electrophysiological examination 8 weeks after the operation in the experimental group showed that incubation period was shorter, and amplitude value was higher, and surrounding connective tissue area of nerve anastomosis was less, and the number of regenerative nerve fibers was large than those in the control group (P<0. 05). Conclusion Medical adhesives and silica gel tube in application of braking nerve anastomosis after peripheral nerve injury can promote the regeneration of nerve fibers and establishment of animal models.关键词
医用胶/周围神经损伤/动物模型/大鼠,Sprague-DawleyKey words
Medical adhesive/Peripheral nerve injuries/Animal models/Rats/Sprague-Dawley分类
医药卫生引用本文复制引用
邹秀强,梁向党,孙赓,余宽宽,房卓群,郭占芳..新型神经吻合口制动动物模型的建立[J].解放军医药杂志,2016,28(4):9-11,3.基金项目
科技部国际合作项目(2014DFA31230) （2014DFA31230）
