河南农业科学2016，Vol.45Issue(3)：106-111,115,7.DOI:10.15933/j.cnki.1004-3268.2016.03.021
汉中主栽柑橘品种对低温胁迫的生理响应
Research on Physiological Response to Low Temperature Stress of Citrus reticulata Cultivars in Hanzhong
摘要
Abstract
Three cultivars of Citrus reticulata ( Fanyatezao, Okitsu, Nidinaul ) planted in Hanzhong of Shaanxi province were used as experimental materials, and their detached leaves were treated at -5 ℃for 3 h,6 h,9 h or 12 h to analyze the physiological responses of Citrus reticulata under low temperature stress. Meanwhile the method of measure parameter and their contribution rate were analyzed using prin-cipal components analysis. The results showed that: the relative electric conductivity of detached leaf all increased gradually in three varieties of Citrus reticulata with the extension of treatment time. The appear-ance order of an obvious increase in three varieties of Citrus reticulata was Fanyatezao>Okitsu>Nichinan 1. The contents of MDA and H2 O2 both showed a gradual upward trend in three varieties of Citrus reticulata. The level ofO2—· increased first and then decreased in leaves in Okitsu and Nichinan 1, while it always showed a upward trend in Fanyatezao. The contents of MDA, O2—· and H2 O2 all showed an order of Fan-yatezao>Okitsu>Nichinan 1 in 3 varieties of Citrus reticulata at the end of treatment time. The changes of proline content exhibited an decrease after the first increase and the contents of soluble sugar increased gradually in three varieties of Citrus reticulata. Compared with the Fanyatezao and Okitsu,the content of osmoregualtion substance elevated levels earlier and more sharply. The activities of SOD,CAT,POD and APX generally increased first and then decreased in Fanyatezao and Okitsu leaves with the extension of treatment time. However, the activities of SOD and APX increased in all the time in Nichinan 1 leaves. Meanwhile CAT and POD activities displayed a decline trend after an initial increase in Nichinan 1 leav-es. The three Citrus reticulata cultivars cold resistance order ( Nichinan 1 >Okitsu >Fanyatezao ) was identified by the average degree of membership hardiness. The results showed that the cumulative contri-bution of REC and proline composite indicator was 97 . 186% and had strong representation as citrus cold analysis of key indicators. The above results indicated that Nichinan 1 had a higher cold resistance than Okitsu and Fanyatezao, because its stronger abilities of osmoregulation and antioxidant activities as well as its littler damages comparing with Okitsu and Fanyatezao under longer low temperature stress.关键词
柑橘/低温胁迫/生理响应/隶属函数/抗寒能力Key words
Citrus reticulata/low temperature stress/physiological response/subordinate function/cold resistance ability分类
农业科技引用本文复制引用
蒋景龙..汉中主栽柑橘品种对低温胁迫的生理响应[J].河南农业科学,2016,45(3):106-111,115,7.基金项目
陕西省科技厅自然科学基础研究计划项目(2014JQ3113) （2014JQ3113）
陕西省教育厅自然科学研究项目(14JK1158) （14JK1158）
陕西理工学院科研基金项目(SLGKY15-41) （SLGKY15-41）
