河南农业科学2016，Vol.45Issue(3)：152-156,5.DOI:10.15933/j.cnki.1004-3268.2016.03.031
响应面法优化山丹鳞茎总黄酮提取工艺的研究
Optimization of Extraction Process of Total Flavonoids from Lilium pumilum bulb Using Response Surface Methodology
摘要
Abstract
The method of response surface analysis was adopted to optimize the extraction conditions of total flavonoids form Lilium pumilum bulb on the basis of single-factor experiment. The results showed that the optimum technological conditions were ethanol concentration of 73%, material-liquid ratio of 32∶1 , reflux temperature of 69 ℃ and reflux time of 120 min. Under such conditions,the actual value of total flavonoids extracted was 4. 685 mg/g,which was basically coincident with the predicted value 4. 729 mg/g. RSD was 0. 661%,indicating that the optimum process conditions were reliable.关键词
山丹/总黄酮/乙醇回流提取/响应面法Key words
Lilium pumilum/total flavonoids/reflux extraction with ethanol/response surface methodology分类
农业科技引用本文复制引用
张昊,邵青,贺晓龙,王延峰..响应面法优化山丹鳞茎总黄酮提取工艺的研究[J].河南农业科学,2016,45(3):152-156,5.基金项目
陕西省高水平大学建设专项资金资助项目(生物学2012SXTS06) （生物学2012SXTS06）
陕西省科技厅农业攻关项目(2012K01-02) （2012K01-02）
延安市科技局项目(2012Ks-13) （2012Ks-13）
