河南农业科学2016，Vol.45Issue(4)：49-53,5.DOI:10.15933/j.cnki.1004-3268.2016.04.011
甘蓝型油菜BnTIR1基因的克隆和表达特征分析
Cloning and Expression Characteristics of BnTIR1 from Brassica napus L.
摘要
Abstract
Transport inhibitor response 1 (TIR1),as an auxin receptor,plays an key role in response to auxin signals. An TIR1 gene,denoted as BnTIR1,was cloned by the rapid amplification of cDNA ends (RACE) from Zhongshuang No. 9. The genomic sequence length of BnTIR1 was 2502 bp,which con-tained two introns. The cDNA sequence length of BnTIR1 was of 2355 bp,which had a 1824 bp ORF en-coding a polypeptide of 607 amino acids. The prediction results indicated that the BnTIR1 protein con-tained conserved AMN1 domain. qRT-PCR results showed the BnTIR1 gene were expressed in roots, stems,shoots,eaves,flowers and siliques,but the transcription level were different among the six tissues, which was the highest in leaves,lowest in flowers;BnTIR1 were induced by drought and ABA,which indi-cated that BnTIR1 could involve in responding to stress.关键词
甘蓝型油菜/克隆/转运抑制应答因子1/ABAKey words
Brassica napus L./cloning/transport inhibitor response 1/ABA分类
农业科技引用本文复制引用
陈帆,谢伶俐,袁程飞,许本波..甘蓝型油菜BnTIR1基因的克隆和表达特征分析[J].河南农业科学,2016,45(4):49-53,5.基金项目
教育部留学回国人员科研启动基金项目(教外司[2014]1685号) （教外司[2014]1685号）
