检验医学与临床2016，Vol.13Issue(8)：1009-1011,1015,4.DOI:10.3969/j.issn.1672-9455.2016.08.001
稳定同位素iTRAQ标记联合液相色谱-串联质谱法定量分析药物性肝损伤患者血清氨基酸的变化
Quantitative analysis of serum amino acids changes in patients with drug-induced liver injury by stable isotope iTRAQ la-beling combined with liquid chromatography tandem mass spectrometry
摘要
Abstract
Objective To explore the changes of serum amino acid levels in the patients with drug‐induced liv‐er injury(DILI) at different stages ,and then to investigate their clinical significance and the biomarkers available for early diagnosis and curative evaluation of DILI .Methods Serum samples from 21 healthy volunteers and 24 patients with different stages( first visiting ,treatment ,outcome) of DILI were collected .The stable isotope iTRAQ labeling combined with liquid chromatography tandem mass spectrometry was adopted to quantitatively detect the serum ami‐no acids and analyzes their differences .Results There were quantified 28 amino acids in serum .Compared with the control group ,14 kinds of amino acids had significant changes in concentrations during the different diagnosis and treatment periods ;among them ,the concentrations of isoleucine ,leucine ,phenylalanine and serine were significantly increased at first visiting ,decreased with the treatment process and gradually close to the normal level .Conclusion Among 4 kinds of amino acids ,The correlation between phenylalanine and DILI is higher ,which might be a potential biomarker for early diagnosis and therapeutic effect evaluation of DILI .关键词
液相色谱-串联质谱/氨基酸/药物性肝损伤/稳定同位素标记Key words
liquid chromatography-tandem mass spectrometry/amino acid/drug-induced liver injury/stable isotope iTRAQ labeling引用本文复制引用
史忱,安卓玲,李鹏飞,刘丽宏..稳定同位素iTRAQ标记联合液相色谱-串联质谱法定量分析药物性肝损伤患者血清氨基酸的变化[J].检验医学与临床,2016,13(8):1009-1011,1015,4.基金项目
国家自然科学基金资助项目（81341015）；北京市自然科学基金资助项目（7142065）。 （）
