检验医学与临床2016，Vol.13Issue(8)：1012-1015,4.DOI:10.3969/j.issn.1672-9455.2016.08.002
B 群链球菌免疫层析法的建立及其临床应用
Establishment of immunochromatographic assay of group B streptococcus and its clinical application
摘要
Abstract
Objective To establish a colloidal gold‐based immunochromatographic assay of group B streptococ‐cus (GBS) and to conduct its clinical application evaluation .Methods Totally 202 vaginal secretion samples were collected .GBS of samples was detected by the bacterial isolation culture method as the gold standard ,meanwhile the GBS colloidal gold strip developed based on the double‐antibody sandwich method was used as control .Its sensitivity and specificity were analyzed .The Kappa value was calculated and the consistence of these two methods was ana‐lyzed .Results The sensitivity and specificity of the immunochromatography method were 95 .60% and 98 .78% re‐spectively ,the total coincidence rate with the bacterial isolation method was 97 .66% ,Kappa was 0 .949 .Conclusion The immunochromatography method has higher sensitivity and higher specificity for detecting GBS ,is simple and ac‐curate ,which has an important clinical application value for the screening and rapid diagnosis of GBS .关键词
B群链球菌/胶体金免疫层析/培养Key words
group B streptococcus/colloidal gold immune chromatography/culture引用本文复制引用
申旺,潘秀华,周剑青,张晓丽,余志华,汤永平..B 群链球菌免疫层析法的建立及其临床应用[J].检验医学与临床,2016,13(8):1012-1015,4.基金项目
广州市科技计划项目（201501010052）。 （）
