检验医学与临床2016，Vol.13Issue(8)：1035-1036,1039,3.DOI:10.3969/j.issn.1672-9455.2016.08.011
肝硬化患者红细胞膜抗溶性增强对淋巴细胞亚群检测的干扰及纠正
Interference and correction of erythrocyte membrane anti-hemolytic enhancement in patients with liver cirrhosis on lym-phocyte subset detection
何东元 1魏崇莉 2柴翠翠 1张文婷 1周文慧 1李丽 1苟莹1
作者信息
- 1. 中国人民解放军第一医院输血科，兰州 730000
- 2. 兰州军区机关门诊部，兰州 730000
- 折叠
摘要
Abstract
Objective To explore the best hemolysis condition of specimen preparation under the condition of specimen anti‐hemolysis increase in the patients with liver cirrhosis .Methods Different doses of hemolysin were used to treat the anti‐hemolysis enhanced specimens from 34 cases of liver cirrhosis in different hemolytic time .The clear degree of WBC scatter diagram under FSC and SSC obtained by cytometry was observed under different hemo‐lytic conditions .Meanwhile the percentages of T ,B and NK lymphocyte subsets were detected .Results The anti‐he‐molysis of erythrocyte membrane in the patients with liver cirrhosis was enhanced .When hemolysin amount was 1 350 μL and the hemolytic time was 10 min ,the classification results of lymphocyte subsets were better .Conclusion In preparing the lymphocyte subset specimen in the patients with liver cirrhosis ,increasing the hemolysin dosage enhances the accuracy of detection results .关键词
肝硬化/淋巴细胞亚群/溶血素/流式细胞术Key words
liver cirrhosis/lymphocyte subset/lysing solution/flow cytometry引用本文复制引用
何东元,魏崇莉,柴翠翠,张文婷,周文慧,李丽,苟莹..肝硬化患者红细胞膜抗溶性增强对淋巴细胞亚群检测的干扰及纠正[J].检验医学与临床,2016,13(8):1035-1036,1039,3.
