临床误诊误治2016，Vol.29Issue(4)：105-109,5.DOI:10.3969/j.issn.1002-3429.2016.04.034
泛素样蛋白D调控乳腺癌干细胞对乳腺癌生物学行为的影响
Effect of Human Ubiquitin D Regulating Breast Cancer Stem Cells on the Biological Behavior of Breast Cancer
摘要
Abstract
Objective To investigate human ubiquitin D ( UBD) expression in breast cancer and its impact on biologi-cal behavior of breast cancer. Methods Histochemical staining was applied to analyze the expression of UBD in breast cancer tissues. Real-time PCR was applied to examine UBD expression in breast cancer cells on genetic level and tumor stem cells. Cell scratch experiments, Transwell experiments and flow cytometry experiments were applied to explore effect of UBD on invasion and metastasis of breast cancer and the malignant behavior of chemotherapy resistance. Spheroid ball experiment and real-time PCR test, under UBD over-expression were applied to further analyze the effects on regulatory phenotype of breast cancer cell lines, and to further investigate whether UBD effected invasion and metastasis of breast cancer and chemotherapy resistance by regulating the stem cells or not. Results UBD high expression levels were detected in breast cancer. UBD promoted malignant biological behavior of breast cancer cells such as invasion, metastasis, and resistance to chemotherapy, UBD promoted enriched population of stem cells in breast cancer cells and self-renewal, overexpression of UBD increased gene expression of CD133, CD90, Nanog, Bmi-1, ABCG2, KLF-4, Oct-4 related with breast cancer stem cells feature (P<0. 05). Conclusion It has been discovered for the first time that UBD molecules are highly expressed in breast cancer tissues. UBD interferes malignant bi-ological behavior of breast cancer cell such as invasion, metastasis, and resistance to chemotherapy. UBD is expected to become a potential clinical marker in diagnosis and treatment.关键词
乳腺肿瘤/泛素化蛋白/肿瘤干细胞/调控Key words
Breast neoplasm/Ubiquitinated protein/Neoplastic stem cell/Regulation分类
医药卫生引用本文复制引用
韩涛,童雅兰,刘军灵,张双鹤,郭放,刘兆喆,谢晓冬..泛素样蛋白D调控乳腺癌干细胞对乳腺癌生物学行为的影响[J].临床误诊误治,2016,29(4):105-109,5.基金项目
科技部十二五重大新药创制平台子课题（2012ZX-09303016-002) （）
2012年辽宁省科技攻关计划课题（2012225019) （）
