中南医学科学杂志2016，Vol.44Issue(1)：11-15,5.DOI:10.15972/j.cnki.43-1509/r.2016.01.003
二甲双胍联合异羟肟酸对骨肉瘤细胞增殖凋亡的影响
Effect of Combining Metformin and Suberoylanilide Hydroxamic Acid on the Proliferation and Apoptosis of Osteosarcoma Cells
摘要
Abstract
Objective To investigate the effect of combining metformin and suberoylanilide hydroxamic acid (SAHA) on the proliferation and apoptosis of human osteosarcoma MG-63 cells. Methods MG-63 cells were treated with metformin and SAHA for 72 h. The proliferation of MG-63 was tested by MTS assay. The capability of colony formation of MG-63 was as-sessed by clonogenicity assay. The cell apoptosis was detected by flow cytometry. The cell cycle and apoptosis associated pro-teins were assessed by Western Blot. Results Metformin or SAHA could inhibit the proliferation of MG-63 cells,respec-tively,and the cell proliferation was inhibited more obviously after combination of theses two drugs. Moreover,combination of two drugs could inhibit colony formation and promote cell apoptosis more significantly. P27,P21,Cyclin D1,Mcl-1,XIAP, Bim,Cytochrome C were significantly changed after the combination therapy. Conclusion Combination of metformin and SAHA could inhibit proliferation and colony formation and promote apoptosis more effectively in MG-63 cells.关键词
二甲双胍/异羟肟酸/骨肉瘤/细胞增殖/细胞凋亡Key words
metformin/suberoylanilide hydroxamic acid/osteosarcoma/proliferation/apoptosis分类
医药卫生引用本文复制引用
申莹莹,苏小桃,欧军,何俊..二甲双胍联合异羟肟酸对骨肉瘤细胞增殖凋亡的影响[J].中南医学科学杂志,2016,44(1):11-15,5.基金项目
国家自然科学基金(81502276) （81502276）
