中南医学科学杂志2016，Vol.44Issue(1)：16-19,4.DOI:10.15972/j.cnki.43-1509/r.2016.01.004
AngⅡ对人脐静脉内皮细胞 CGRP 受体组分的调节差异
Difference of Expression of CGRP Receptor Components Regulated by Angiotensin Ⅱ in Human Umbilical Vein Endothelial Cells
摘要
Abstract
Objective To investigate the effect of angiotensin Ⅱ on the expression of calcitonin gene related peptide (CGRP) in human umbilical vein endothelial cells (HUVECs). Methods HUVECs was employed as the experimental object,cells were incubated by free serum for 24 h for synchronous cell cycle,then,Ang Ⅱ group cells were incubated with different concentration of angiotensin Ⅱ (Ang Ⅱ),the control cells were incubated by free Ang Ⅱ. The cell proliferation and apoptosis were observed by flow cytometry. Western blot was used to determine the expressions of calcitonin receptor like receptor (CRLR),receptor activity modifying protein-1 (RAMP1) and receptor component protein (RCP). Results Compared with the control group,in the treated cells,Ang Ⅱ inhibited the HUVECs proliferation and induced cell apopto-sis,and also up-regulated the CRLR expression of HUVECs (P < 0. 01) as well. In addition to the other two receptor pro-teins,Ang Ⅱ (0. 01 ~ 100 nmol/ L)down regulated expression of RAMP1 and RCP expression (P < 0. 05) in a dose-de-pendent manner. Conclusion Ang Ⅱ can affect the expression of CGRP receptor in HUVECs,which can cause the functional changes of CGRP receptor in the cells.关键词
血管紧张素Ⅱ/降钙素基因相关肽受体/人脐静脉内皮细胞Key words
angiotensin Ⅱ/calcitonin gen related-peptide receptor/human umbilical vein endothelial cell分类
医药卫生引用本文复制引用
吴媛,彭虹艳,朱怡,骆竟妃,秦旭平..AngⅡ对人脐静脉内皮细胞 CGRP 受体组分的调节差异[J].中南医学科学杂志,2016,44(1):16-19,4.基金项目
国家自然科学基金项目(No.81173060,81500041) （No.81173060,81500041）
