中南医学科学杂志2016，Vol.44Issue(1)：20-24,5.DOI:10.15972/j.cnki.43-1509/r.2016.01.005
阿托伐他汀对大鼠脑缺血再灌注 PERK/elfR2a通路及 Caspase-3表达的影响
The Study of PERK/eIF2a Pathway and the Expression of Caspase-3 in Ischemia-reperfusion Rats and Atorvastatin Intervention
摘要
Abstract
Objective To study the PERK/ eIF2a pathways and Caspase 3 in the mechanism of action of ischemia reperfusion injury in rats and the effect of atorvastatin. Methods Produce the ischemia reperfusion model rats by Middle cerebral artery embolism method which were divided into the ischemia reperfusion group,control group,atorvastatin interven-tion group and eIF2a suppression group. To observe the changes of ischemic brain,specimens were treated with TTC stai-ning,the PERK,Caspase-3 protein expression and protein phosphorylation eIF2a were detected by western-blot. Results Compared with the control group,after ischemia reperfusion,PERK protein expression and protein phosphorylation eIF2a in-creased,the expression of Caspase 3 was enhanced,and the expression of PERK protein and phosphorylation eIF2a was re-lieved after atorvastatin intervention. The expression of Caspase-3 and phosphorylation eIF2a was inhibited by inhibitor Salu-brinal,which had no effect no PERK. Large cerebral infarction in ischemia reperfusion group was visible by TTC staining af-ter ischemia reperfusion. Cerebral infarction volume was significantly narrowed by atorvastatin intervention or inhibitor Salu-brinal. Conclusion Cell apoptosis was related to the PERK/ eIF2a / Caspase 3 pathways after endoplasmic reticulum stress,atorvastatin can reduce the damage of cerebral ischemia reperfusion.关键词
蛋白激酶 R 样内质网激酶/eIF2a/内质网应激/Caspase-3/局灶性脑缺血再灌注/阿托伐他汀Key words
protein kinase-like ER kinase/EIF2a/endoplasmic reticulum stress/Caspase-3/ischemia reperfusion/atorvastatin分类
医药卫生引用本文复制引用
彭文娟,杨剑文,刘湘玉,杨期明..阿托伐他汀对大鼠脑缺血再灌注 PERK/elfR2a通路及 Caspase-3表达的影响[J].中南医学科学杂志,2016,44(1):20-24,5.基金项目
湖南省卫生计生委资助项目(B2015-73) （B2015-73）
