中南医学科学杂志2016，Vol.44Issue(1)：25-28,47,5.DOI:10.15972/j.cnki.43-1509/r.2016.01.006
甲状腺素对非酒精性脂肪肝大鼠 ANGPTL3表达的影响
Effects of Thyroid Hormone on the Expression of ANGPTL3 in Nonalcoholic Fatty Liver in Rats
摘要
Abstract
Objective To investigate the effects of TH on the expression of ANGPTL3 of NAFLD rats,and the effect of TH on hepatic steatosis in NAFLD rats by ANGPTL3 pathway. Methods 30 SD male rats,were randomly divided into 5 groups:normal control group,high fat group,thyroxine prevention group,treatment group,gemfibrozil group;All rats were fed with high fat diet except the normal group,blood was taken out from the tail of rat at 0,4,8,12 weeks,All of them were killed at the end of 12 weeks,the degree of hepatic steatosis score was observed by HE staining of Liver pathological sec-tion. Serum ANGPTL3 content was detected by ELISA method,and the mRNA expression of ANGPTL3 in liver tissue was measured with RT-PCR. Results Rat liver steatosis degree in thyroxine prevention group and gemfibrozil group was low-er than the high fat group and thyroxine treatment(P < 0. 05). In the high fat group,the serum level of ANGPTL3 was in-creased at 8W,the expression of ANGPTL3 mRNA in liver tissue was significantly higher than the normal control( P <0. 01). Thyroxine prevention group rats showed the decrease in serum concentration of ANGPTL3 at 8 weeks,12 weeks,and lower hepatic ANGPTL3 mRNA level,compared with high cholesterol group, gemfibrozil group and thyroxine treatment groups,was statistically significant(P < 0. 05). Thyroxine treatment group and gemfibrozil group can also in a certain degree decreasing serum ANGPTL3 level and expression of ANGPTL3 mRNA in liver tissue(P < 0. 05),but the extent of reduction is less than thyroxine prevention group(P < 0. 05). Conclusion The formation process of NAFLD increased with AN-GPTL3,ans early administration of small dose of TH hepatic adipose degeneration in NAFLD rats improved through AN-GPTL3 pathway.关键词
非酒精性脂肪肝/血管生成素样蛋白 3/甲状腺激素Key words
nonalcoholic fatty liver disease/angiopoietin-like protein 3/thyroid hormones分类
医药卫生引用本文复制引用
程静屏,傅念,罗艳云,马娜,阳学风..甲状腺素对非酒精性脂肪肝大鼠 ANGPTL3表达的影响[J].中南医学科学杂志,2016,44(1):25-28,47,5.基金项目
本项目由2012年武汉市卫生计生委科研基金支持(编号 WX12B07) （编号 WX12B07）
