中南医学科学杂志2016，Vol.44Issue(1)：29-33,5.DOI:10.15972/j.cnki.43-1509/r.2016.01.007
Apelin-13通过 AMPK 通路抑制同型半胱氨酸诱导的内皮细胞损伤
Apelin-13 Inhibits the Damage of Endothelial Cells Induced by Homocysteine via AMPK Pathway
摘要
Abstract
Objective To observate the protective effect of Apelin-13 on homocysteine (Hcy)-induced damage of vascular endothelial cells and to explore the possible mechanism. Methods Human umbilical vein endothelial cells (HUVECs) were incubated with 10 mmol/ L Hcy to induce damage. After pretreatment with Apelin-13 (0. 1,1. 0 and 10. 0 μmol/ L) for 1 h,the protective effect of Apelin-13 was investigated. MTT assay was used to detect the survival rate of cells. The level of lactate dehydrogenase (LDH) in the medium was measured by colorimetry. The expression of Amp activa-ted protein kinase (AMPK) in HUVECs was measured by Western Blot. Results Compared with the control group,the survival rate of cells was singificantly decreased,the level of LDH in the medium was singificantly increased in the Hcy group (all P < 0. 05). Compared with the Hcy group,the survival rate of cells was singificantly increased,the level of LDH in the medium was singificantly decreased in the Apelin-13 (1. 0 and 10. 0 μmol / L) groups (all P < 0. 05). Compared with the control group,the expressions of p-AMPK in the Hcy group did not have singificant difference (P > 0. 05). Com-pared with the Hcy group,the expressions of p-AMPK in Apelin-13 (1. 0 and 10. 0 μmol / L) groups were singificantly up-regulated (all P < 0. 05). The endothelial cell protection of Apelin-13 was partly abolished by the inhibitor of AMPK Com-pound C. Conclusion Apelin-13 inhibits the damage induced by homocysteine in HUVECs,which the mechanisms may be related to the up-regulation of AMPK phosphorylation.关键词
Apelin-13/腺苷酸活化蛋白激酶/同型半胱氨酸/内皮细胞Key words
Apelin-13/Amp activated protein kinase/homocysteine/endothelial cells分类
医药卫生引用本文复制引用
王波,周洁,张涛,李国术,曾高峰..Apelin-13通过 AMPK 通路抑制同型半胱氨酸诱导的内皮细胞损伤[J].中南医学科学杂志,2016,44(1):29-33,5.基金项目
衡阳市科技计划项目(2011KJ49) （2011KJ49）
