南京林业大学学报（自然科学版）2016，Vol.40Issue(2)：33-40,8.DOI:10.3969/j.issn.1000-2006.2016.02.006
柽柳 GF14基因的克隆与表达分析
Cloning and expression analysis of ThGF14 gene in Tamarix hispida
摘要
Abstract
The ubiquitous family of 14-3-3 ( also known as GF14) proteins function as regulators in a variety of stress response processes in plants. Five GF14 genes from Tamarix hispida, designated ThGF14a to ThGF14 e, were identified from an exhaustive search of six cDNA library databases. The basic bioinformatics analysis of five GF14 genes were im⁃plemented, and the expression patterns of GF14 genes were analyzed by qRT-PCR under the treatment of stresses. The results were as follows:Bioinformatics analysis showed that ThGF14 proteins contained a highly conserved 14-3-3 do⁃main, and the amino acid sequences are highly conserved except at the N-and C-terminal regions. A phylogenetic anal⁃ysis indicated that ThGF14 proteins have bothε-like and non-εforms. The expression profiles of the five ThGF14 genes in response to NaCl, polyethylene glycol (PEG), low temperature, and CdCl2 for various treatment periods (6, 24, 48, and 72 h) in leaf and root tissues were investigated using qRT-PCR. The results showed that most of the five ThGF14 genes were induced (>2-fold) in leaf and root tissues under abiotic stress, and there were significant differences in re⁃sponse to stress between the various genes. Interestingly, the ThGF14c gene, which generated analogous results under stress in both tissues, was the most highly induced of expression level. Taken together, these results indicate that Th⁃GF14 genes are integral to the abiotic stress response.关键词
柽柳/ThGF14/生物信息学分析/基因表达/非生物胁迫Key words
Tamarix hispida/ThGF14/bioinformatics/gene expression/abiotic stress分类
农业科技引用本文复制引用
宁坤,宋鑫,李慧玉..柽柳 GF14基因的克隆与表达分析[J].南京林业大学学报（自然科学版）,2016,40(2):33-40,8.基金项目
中央高校基本科研业务费专项资金项目 （）
