南京林业大学学报（自然科学版）2016，Vol.40Issue(2)：41-47,7.DOI:10.3969/j.issn.1000-2006.2016.02.007
白桦BpGT14基因表达模式及对非生物胁迫诱导的响应
The expression patterns of BpGT14 gene in Betula platypylla Suk. and the response to biotic stress
摘要
Abstract
Birch(Betula platypylla Suk.) GT14 gene sequence, named BpGT14 gene (JQ409354) have been cloned in this study. Bioinformatics was used to analyze its physical and chemical properties. The expression of BpGT14 gene in xy⁃lem, phloem, leaves and male inflorescence of wild birch was analyzed through real⁃time PCR. At the same time, we had treated suspension cells of birch stems with salicylic acid ( SA) , salt stress ( NaCl) , cadmium ( CdCl2 ) and 4℃ to determine the stress response of BpGT14. Research indicated that the gene was 1 302 bp, encoding 433 amino acids. Protein analysis showed that the BpGT14 gene contained important domain of acetyl glucosaminyl transferase which be⁃longs to a glycosyltransferase 14 family. The GT14 protein we selected from different species showed highly conserved a⁃mino acid sequence in the 100-350 region and the identity of BpGT14 and Populus trichocarpa GT14 was up to 79%. BpGT14 gene real⁃time quantitative PCR results showed that the gene expression had specificity in different months and parts. Meanwhile, the results showed that the expression levels of BpGT14 gene was higher in xylem, phloem and leaves than that in male inflorescence. The results of abiotic stress treatment showed that BpGT14 gene was respond to abiotic stress, but its response pattern was different. The results of SA,CdCl2 and 4 ℃ treatments showed that early treatment increased the expression of the target gene. After 6 h treatment, the expression of BpGT14 gene was 51.2, 48.9 and 3.3 times compared with the control group, but then the relative expression levels were lower compared with the control group except the 4℃ at 96 h. NaCl treatment had been showing downward trend and reduced by 93.7% compared with control in 24 h. This study was to reveal the molecular structure of BpGT14, figure out the expression patterns of BpGT14 and the response to biotic stress and explore the function of BpGT14 in birch growth and development. It provides the basis and inspiration for the research of other woody plants.关键词
白桦/糖基转移酶/BpGT14/非生物胁迫/表达模式Key words
birch (Betula platypylla Suk.)/glycosyltransferase/BpGT14/abiotic stress/expression patterns分类
农业科技引用本文复制引用
李蕾蕾,孙丰坤,董恒,詹亚光,杨丹,曾凡锁..白桦BpGT14基因表达模式及对非生物胁迫诱导的响应[J].南京林业大学学报（自然科学版）,2016,40(2):41-47,7.基金项目
国家自然科学基金项目（31200463，J1210053）；中央高校基本科研业务费专项资金项目 （）
