南京林业大学学报（自然科学版）2016，Vol.40Issue(2)：59-64,6.DOI:10.3969/j.issn.1000-2006.2016.02.010
不同活性炭对杂交鹅掌楸体胚发生的影响
Effects of different activated carbon on somatic embryogenesis of Liriodendron hybrids
摘要
Abstract
Activated carbon is often used to improve cell growth and development in somatic embryogenesis. In this stud⁃y, three types of activated carbon, including phosphoric acid activated carbon, activated carbon for refinement of injec⁃tion and activated carbon purchased from Sigma were added respectively to the mediums for improving somatic embryo⁃genesis frequency of two genotypes of cell lines in Liriodendron hybrids. The number of total somatic embryos and cotyle⁃don embryos were counted, pH and osmotic pressure of the mediums were detected, and the adsorption ability of activa⁃ted carbon were analyzed to select the most suitable activated carbonl and to find their potential effects on somatic embry⁃ogenesis of Liriodendron hybrids. The results showed that the first two types of activated carbon induced more total somat⁃ic embryos and cotyledon embryos than the third type, especially the activated carbon for refinement of injection. The ad⁃sorption ability of activated carbon for refinement of injection was moderate among the three, and the pH and osmotic pressure of the mediums containing it were also temperate compared with the other two types of activated carbon. It was concluded from the results that the adsorption ability of activated carbon may change the pH and osmotic pressure of me⁃diums directly to influence the somatic embryogenesis of Liriodendron hybrids.关键词
杂交鹅掌楸/体胚发生/活性炭Key words
Liriodendron hybrids/somatic embryogenesis/activated carbon分类
农业科技引用本文复制引用
鲁路,陆叶,盛宇,郑晨,施季森,陈金慧..不同活性炭对杂交鹅掌楸体胚发生的影响[J].南京林业大学学报（自然科学版）,2016,40(2):59-64,6.基金项目
国家高技术研究发展计划（2013AA102705）；国家自然科学基金项目（31170619）；教育部新世纪优秀人才支持计划；江苏高校优势学科建设工程资助项目 （）
