日用化学工业2016，Vol.46Issue(3)：168-172,5.DOI:10.13218/j.cnki.csdc.2016.03.010
蚯蚓小分子多肽提取物对人皮肤细胞活性及胶原分泌的影响
Effect of low molecular weight peptides extract from Earthworm on cell viability and synthesis of collagen in cultured human skin cells
摘要
Abstract
The effect of low molecular weight peptides extract from Earthworm (LMWPEE)on cell viability in cultured normal adult human skin fibroblasts (HSF)and normal adult human epidermal keratinocytes (HEK) was studied,separately. Its effect on synthesis of type I procollagen in cultured HSF was also studied. The results showed that,as comparing with the control group,the LMWPEE with mass concentrations between 25 and 400 mg/L significantly increases cell viability of HSF and HEK,but it has no effect on cell cycle of HSF and HEK. The effect of LMWPEE on HSF cell viability shows a certain dose-effect relationship,while best dosage of LMWPEE for HEK cell viability is 50 mg/L. LMWPEE remarkably increases the production of type I procollagen in cultured HSF. The production of type I procollagen increases by 31%(P <0. 05 )and 51%(P<0. 01 ) at mass concentration 200 and 400 mg/L of LMWPEE as compared with control group, respectively.关键词
化妆品添加剂/蚯蚓/小分子多肽/人皮肤成纤维细胞/人表皮角质形成细胞/Ⅰ型前胶原蛋白Key words
cosmetic additives/Earthworm/low molecular weight peptides/human skin fibroblasts/human epidermal keratinocytes/type I procollagen分类
化学化工引用本文复制引用
刘廷强,严泽民,侯冬梅,周华峰,段明星..蚯蚓小分子多肽提取物对人皮肤细胞活性及胶原分泌的影响[J].日用化学工业,2016,46(3):168-172,5.基金项目
2014年度江苏省博士后科研计划资助项目 （）
