日用化学工业2016，Vol.46Issue(3)：178-182,5.DOI:10.13218/j.cnki.csdc.2016.03.012
紫外可见光谱分析海娜粉中的指甲花醌
UV-vis spectral analysis of lawsone in henna powder
摘要
Abstract
UV - vis spectra of henna aqueous extract and lawsone solution of different pH mediums were analyzed to select the detection wavelength and the reference solution for determining lawsone content in henna powder by spectrophotometer. Selecting 453 nm as the detection wavelength,with henna powder extract in hydrochloric acid of pH value 2 as the reference solution,the absorbance value of the testing solution samples in NaOH solution of pH =12 as the working medium,the lawsone mass concentration in the henna powder sample was quantified based upon comparison with the standard calibration curve. The results showed that within the lawsone mass concentration range of 10~140 mg/L,the absorbance follows Lambert-Beer's law;the linear correlation coefficient is r=0. 999 95 and the precision RSD is less than 0. 47%. The absorbancevalue of the sample solution can keep stable within 14 h,while the lawsone spiked recoveries achieves 99. 0% ~100. 0%. The method is simple,reliable,and can be used to detect the lawsone content in henna powder.关键词
海娜粉/指甲花醌/紫外可见光谱Key words
henna powder/lawsone/UV-vis spectrum分类
化学化工引用本文复制引用
乔仙蓉..紫外可见光谱分析海娜粉中的指甲花醌[J].日用化学工业,2016,46(3):178-182,5.基金项目
山西工程职业技术学院教科研课题资助项目 （）
