肿瘤预防与治疗2016，Vol.29Issue(1)：1-4,4.DOI:10.3969/j.issn.1674-0904.2016.01.001
过表达Numb基因对人膀胱癌5637细胞周期和增殖的影响
Effect of Numb Gene on Cell Cycle and Proliferation in Human Bladder Cancer 5637 Cells
摘要
Abstract
Objective:To study the effect of Numb gene on cell cycle and proliferation in human bladder cancer cells and its related mechanism.Methods:Human bladder cancer 5637 cells which were transfected by the Numb-ORF plasmid were used as the experimental group.The negative control and blank control were also set.The expression of Numb or Cyclin D1 was detected by Real-Time PCR and Western Blot.The cell cycle was analyzed by Flow cytometry.Cell pro-liferation was assessed by comparing with absorbance at 490nm using a Micro-plate Reader.Results:Compared with the negative control (5.66 ±0.53)and blank control (5.81 ±0.47),the △CT value of Numb in the Numb-ORF group (1.58 ±0.41)was significantly lower (F =77.39,P =0.00).Meanwhile,the △CT value of Cyclin D1 in the Numb-ORF group (4.92 ±0.73)was significantly higher than that in the negative control (2.59 ±0.33)and blank control (2.45 ± 0.26)(F =11.70,P =0.01).The ratio (%)of G0 /G1 cells was as follows:Numb-ORF group was 49.76 ±7.07,nega-tive control was 36.52 ±3.32 and blank control was 38.21 ±2.06 (F =7.17,P =0.03).In proliferation assay,compared with negative control (0.52 ±0.06)and blank control (0.55 ±0.04),the OD value at 24h in Numb-ORF group (0.35 ± 0.08)was significantly reduced (F =9.03,P =0.02).Conclusion:Numb gene could promote G0 /G1 phase arrested and inhibit proliferation of bladder cancer cells,the suppressive effects may be due to down-regulation of Cyclin D1.关键词
膀胱肿瘤/Numb 基因/细胞周期蛋白 D1Key words
Bladder Neoplasm/Numb Gene/Cyclin D1分类
医药卫生引用本文复制引用
司马晋,张保,艾青,王保军,马鑫,张旭..过表达Numb基因对人膀胱癌5637细胞周期和增殖的影响[J].肿瘤预防与治疗,2016,29(1):1-4,4.基金项目
国家自然科学基金 （）
