山东农业科学2016，Vol.48Issue(4)：1-7,7.
肥城桃果实 VDAC 基因的克隆、生物信息学分析及蛋白诱导表达
Clone,Bioinformatics Analysis and Protein Induced Expression of VDAC Gene in Feicheng Peach Fruits
摘要
Abstract
Mitochondrial membrane permeability transition pore (MPTP)is a kind of protein complex, and plays important roles in cell mitochondria -mediated apoptosis.As an important part of MPTP,the volt-age -dependent anion channel protein (VDAC)regulates the opening of MPTP.However,its regulation mechanism in plant is still unclear.In this study,a cDNA encoding VDAC protein was isolated from Feicheng peach fruits by RT -PCR and rapid amplification of cDNA ends (RACE).The full -length cDNA of VDAC was 1 225 bp and contained 828 bp of coding sequence encoding a polypeptide of 276 amino acids.Multiple sequence alignment and phylogenetic tree analysis showed that the deduced protein VDAC of peach shared higher identify with that from other plants.The recombinant protein pET -30a -VDAC was constructed in vitro,expressed in Escherichia coli BL21,and purified through Ni -chelating affinity chromatography.The re-sults would lay a foundation for studying the structure and function of VDAC protein in peach fruits in the next step.关键词
肥城桃/线粒体/电压依赖型阴离子通道蛋白(VDAC)/基因克隆/生物信息学分析/蛋白表达Key words
Feicheng peach/Mitochondria/Voltage -dependent anion channel protein (VDAC)/Gene clone/Bioinformatics analysis/Protein expression分类
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衣冠帅,刘雨辰,朱树华..肥城桃果实 VDAC 基因的克隆、生物信息学分析及蛋白诱导表达[J].山东农业科学,2016,48(4):1-7,7.基金项目
国家自然科学基金项目 （）
