山东农业科学2016，Vol.48Issue(4)：8-13,6.DOI:10.14083/j.issn.1001-4942.2016.04.002
水稻α－NAC 基因家族的克隆及生物信息学分析
Molecular Cloning and Bioinformatic Analysis of α-NAC in Rice
摘要
Abstract
Nascent polypeptide -associated complex (NAC)is a heterodimer consisting of αand βsub-units.There are three members of α-NAC in rice genome,which are localized in chromosome 1,3 and 5 re-spectively.The full open reading frames of the three α-NAC genes were cloned by RT -PCR from Oryza sati-va cv.Zhonghua 11.Further bioinformatics analysis revealed that the amino acid sequence of Osα-NAC was conserved in NAC and UBA domains,and showed the highest similarity to ZmNACs.Os1gNAC shared 67.4% and 81.8% homology with Os3gNAC and Os5gNAC respectively,whereas Os3gNAC and Os5gNAC shared 69.5% homology.These results would provide references for protein purification and structural biologi-cal study,and lay a foundation for functional analysis of Osα-NAC.关键词
水稻/新生多肽结合复合体 α链/生物信息学分析Key words
Rice/αchain of nascent polypeptide -associated complex/Bioinformatics analysis分类
农业科技引用本文复制引用
王晓静,孙林静,马忠友,孙癑,李军玲,张融雪,闫双勇,刘学军..水稻α－NAC 基因家族的克隆及生物信息学分析[J].山东农业科学,2016,48(4):8-13,6.基金项目
国家科技支撑计划项目（2013BD0508）；农业部转基因专项（2011ZX08001－004）；天津市农业科学院院长基金项目 （）
