山东农业科学2016，Vol.48Issue(4)：14-17,4.DOI:10.14083/j.issn.1001-4942.2016.04.003
利用特异性 SNP 位点鉴定花生杂交 F1代真假杂种
Identification of True F1 Peanut Hybrid with Specific SNP Locus
摘要
Abstract
The peanut varieties,Luhua 14,Tifrunner and Silihong,were used as parents to make hybrid combinations through reciprocal cross.A set of technology for identifying the true F1 hybrid by restriction en-zyme digestion was established using the special SNP loci of parents.The results showed that the digestion band patterns of true hybrids were consistent with those of parents,while false hybrids only showed the female parent band patterns.The true hybrid rate of the four combiniations ranged from 10.5% to 36.7%.There-fore,this technology could be used to identify the true F1 hybrid of peanut effectively.关键词
花生/SNP/杂种鉴定/限制性内切酶Key words
Peanut/SNP/Hybrid identification/Restriction enzyme digestion分类
农业科技引用本文复制引用
韩燕,马登超,刘译阳,崔凤,孙秀芹,李荣冲,万书波,李国卫..利用特异性 SNP 位点鉴定花生杂交 F1代真假杂种[J].山东农业科学,2016,48(4):14-17,4.基金项目
国家科技支撑计划项目（2014BAD11B00）；山东省农业良种工程（2013LZ01）；山东省农业科学院青年拔尖人才计划专项；山东省农业科学院院地科技合作引导计划 （）
