山东农业科学2016,Vol.48Issue(4):21-23,3.DOI:10.14083/j.issn.1001-4942.2016.04.005
萝卜离体再生体系的建立
Establishment of in vitro Regeneration System for Radish
摘要
Abstract
To select the best hormone combination for inducing adventitious shoots and adventitious roots,the in vitro culture of cotyledons with petiole from 8 genotypes of radish were carried out in this research.The results showed that Xinuoxiaoqing and Cuilüradish had high -frenquency regeneration potential.The optimum medium for their adventitious shoot induction was MS +30 g·L -1 Sucrose +0.7% Agar +5 mg·L -1 6 -BA +0.05 mg·L -1 NAA,and that for adventitious root induction was MS +30 g·L -1 Sucrose +0.7% Agar +5 mg·L -1 6 -BA +0.10 mg·L -1 NAA.关键词
萝卜/愈伤组织/不定芽/不定根/激素Key words
Radish/Callus/Adventitious shoot/Adventitious root/Hormone分类
农业科技引用本文复制引用
黄俊豪,徐文玲,刘贤娴,刘辰,王淑芬,杨建平..萝卜离体再生体系的建立[J].山东农业科学,2016,48(4):21-23,3.基金项目
“十二五”国家科技支撑计划项目(2012BAD02B01-6、2013BAD01B04-18);山东省现代农业产业技术体系项目 ()