中国生态农业学报2016，Vol.24Issue(4)：470-477,8.DOI:10.13930/j.cnki.cjea.151257
13C 标记磷脂脂肪酸分析在土壤微生物生态研究中的应用*
Applicationof13C-labeledPLFAanalysisinsoilmicrobialecologystudies
摘要
Abstract
Analysisofphospholipidfattyacids(PLFA)isaneffectivenon-culture-basedtechniqueforinformationonlivingsoilmicrobialcommunity.PLFAisanimportantcomponentofmicrobialcellmembranes.MicrobescansynthesizethisspecialPLFAthroughvariousbiochemicalpathwayshencesomePLFAcanbeusedasbiomarkerforamicrobialcommunity.Whencoupledwithstableisotope13C-labeled(13C-PLFA)technique,PLFAcanbeusedtoidentifyinsitustructuresofsoilmicrobialcommunities.Thiscanalsobeusedtoexploresoilmicrobialfunctionalcommunitiesresponsibleformetabolicprocessesofsoilcarbonsourcesinsoilecosystem,thusprovidingabundantinformationaboutmicrobialinteractionsincomplexcommunities.Thistechniquehasthepotentialforwidefutureapplications.Theprincipleof 13C-PLFAtechniqueisasfollows:1)adding13C-rich substrates to the soils assimilated by some members of the soil microbial community; 2) extracting and purifyingPLFAfromsoilsubstratemixtures;3)determining13CvalueofPLFAusinggaschromatographycombustionisotoperatiomassspectrometry(GC-C-IRMS);and4)derivingrequiredinformationbycomparativeanalysis.Thisstudyintroducedtheprincipleof 13C-PLFA technique and reviewed the applications of 13C-PLFA in the fields of photosynthetic carbon utilization byrhizospheremicroorganisms,primingeffectsofsoilorganicmatterdecompositionandmineralization,microbialoxidationofmethane,degradationoforganicpollutantsandmicrobialutilizationofexogenoussimpleandcomplicatedcarbonsources.Thelimitationsandapplicationpotentialsofthistechniquewerealsodiscussed.Itwasconcludedthat 13C-PLFAmethodwasanexcellentwayofprovidinginsightintotherelationsbetweenmicrobialcommunitycompositionandsoilbiogeochemicalcycle.关键词
磷脂脂肪酸/稳定性同位素标记技术/土壤微生物/群落结构/微生物功能Key words
Phospholipidfattyacid/Stableisotopelabeledtechnique/Soilmicrobe/Communitystructure/Microbialfunction分类
农业科技引用本文复制引用
李增强,赵炳梓,张佳宝..13C 标记磷脂脂肪酸分析在土壤微生物生态研究中的应用*[J].中国生态农业学报,2016,24(4):470-477,8.基金项目
国家自然科学基金项目(41271311)、国家科技支撑计划项目(2012BAD05B0203)和中国科学院科技服务网络计划(STS 计划)项目(KFJ-SW-STS-142-3,KFJ-EW-STS-083-2,KFJ-EW-STS-055-1)资助*The study was supported by the National Natural Science Foundation of China(No.41271311), the National Key Technologies R&DProgramofChina(2012BAD05B0203)andtheScienceandTechnologyServiceNetworkInitiativeProgramofChineseAcademyofSciences(No.KFJ-SW-STS-142-3,KFJ-EW-STS-083-2,KFJ-EW-STS-055-1). （41271311）
